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New uses of tauroursodeoxycholic acid

A technology of tauroursodeoxycholic acid and drugs, applied in the field of biomedicine, can solve the problems that drugs cannot meet the needs of treatment, etc., achieve the effects of preventing or treating viral infections, increasing the scope of application, and achieving excellent effects

Inactive Publication Date: 2016-10-19
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For the above-mentioned diseases, the medicines in the prior art still cannot meet the needs of treatment

Method used

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  • New uses of tauroursodeoxycholic acid
  • New uses of tauroursodeoxycholic acid
  • New uses of tauroursodeoxycholic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0157] Example 1: TUDCA can preventively inhibit the death of human type II alveolar epithelial cell line A549 cells caused by H5N1 type avian influenza virus infection

[0158] Experimental Materials:

[0159] (1) Main experimental instruments: three-level biological safety laboratory, three-level biological safety cabinet, pipette, pipette, CORNING company cell culture consumables, etc.

[0160] (2) Main experimental reagents: TUDCA (T0266, purchased from Sigma-Aldrich Company), cell proliferation and toxicity detection kit (MTS, Cell titer 96 Aqueous One Solution, Cell Proliferation Assay, Promega).

[0161] (3) Virus: H5N1 avian influenza virus A / Jilin / 9 / 2004 (H5N1).

[0162] (4) Experimental cells: human type II alveolar epithelial cell line A549 cells (purchased from Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences), F-12 / HAM's medium (purchased from Hyclone), fetal bovine serum (26140-079, purchased from Invitrogen Company), digestive juice (0....

Embodiment 2

[0180] Example 2: TUDCA can preventively and therapeutically reduce the mortality of human type II alveolar epithelial cell line A549 caused by H5N1 avian influenza virus infection

[0181] Experimental Materials:

[0182] (1) Main experimental instruments: three-level biological safety laboratory, three-level biological safety cabinet, pipette, pipette, CORNING company cell culture consumables, etc.

[0183] (2) Main experimental reagents: TUDCA (T0266, purchased from Sigma-Aldrich Company), cell proliferation and toxicity detection kit (MTS, Cell titer 96 Aqueous One Solution, Cell Proliferation Assay, Promega).

[0184] (3) Virus: H5N1 avian influenza virus A / Jilin / 9 / 2004 (H5N1).

[0185] (4) Experimental cells: with embodiment 1;

[0186] experimental method:

[0187] (1) A549 cells are cultured on F-12 / HAM's medium, which contains 10% fetal bovine serum, 100U / ml of penicillin and 100mg / ml of streptomycin in the F-12 / HAM's medium, and then placed in CO 2 In an incubato...

Embodiment 3

[0201] Example 3: TUDCA can reduce the death rate of mice caused by H5N1 avian influenza virus infection

[0202] Experimental Materials:

[0203] (1) Main experimental instruments: three-level biosafety laboratory, three-level biological safety cabinet, animal breeding cabinet, mouse breeding cage, small animal surgical instruments, sterile syringes, pipettes, and pipettes.

[0204] (2) Main experimental reagents: TUDCA (T0266, purchased from Sigma-Aldrich Company), contrast solvent PBS, 1% (W / V) pentobarbital sodium solution, virus diluent, disinfectant (2.5% iodine and 75% alcohol).

[0205] (3) Virus: H5N1 avian influenza virus A / Jilin / 9 / 2004 (H5N1).

[0206] (4) Experimental animals: SPF grade wild-type (WT) C57BL / 6J mice (4 weeks old) (purchased from China Institute for the Control of Pharmaceutical and Biological Products).

[0207] experimental method:

[0208] (1) Grouping:

[0209] Uninfected group: virus control + solvent control group, virus control + drug group...

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Abstract

The present invention relates to the technical field of biomedicine, and mainly provides new uses of tauroursodeoxycholic acid, wherein the new uses comprise that the tauroursodeoxycholic acid inhibits the virus-entry-host cells adopting endocytosis so as to achieve prevention and treatment of virus infection, anti-injury, and anti-fibrosis. The present invention provides applications of the tauroursodeoxycholic acid in preparation of drugs for treatment of acute lung injury and pulmonary fibrosis, such that the application range of the tauroursodeoxycholic acid is broadened, and the new drug selection is provided for the virus infection patients. According to the present invention, it is confirmed that the tauroursodeoxycholic acid can effectively prevent and treat H5N1 avian influenza virus, human adenovirus and Zaire-type Ebola virus, and it is cleared that the pulmonary fibrosis prevention and control effect of the tauroursodeoxycholic acid is superior to the pulmonary fibrosis prevention and control effect of the chloroquine.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a new application of tauroursodeoxycholic acid. Background technique [0002] The chemical name of tauroursodeoxycholic acid (TUDCA) is 3α, 7βdihydroxycholanoyl-N-taurine, which is formed by shrinking the carboxyl group of ursodeoxycholic acid (UDCA) and the amino group of taurine. Conjugated bile acids. TUDCA is white crystal, easily soluble in water. TUDCA was first discovered in bear bile in 1902, and it is the iconic active ingredient in bear bile. Bear bile is the treasure of traditional Chinese medicine, the first of all gallbladders, and the first of the four major animal medicinal materials in China. It has a history of thousands of years of medicinal use and is known as the "gold in medicine". "Compendium of Materia Medica" records: Bear bile, bitter in taste, cold in nature, non-toxic, can clear away heat, calm the liver, benefit gallbladder, improve eyesight, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/56A61P31/16A61P31/12A61P31/14A61P11/00
Inventor 蒋澄宇李宁金宁一张延旭钟颖朱锦东
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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