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KCK polypeptide modified gold nanocluster and preparation method thereof

A gold nanocluster and polypeptide modification technology, which is applied in the field of red fluorescent gold nanoclusters, can solve the problems of high production cost, complex production process, and high toxicity of cell imaging, and achieve good fluorescence, strong operability, and low toxicity.

Active Publication Date: 2016-10-12
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art, to seek a KCK polypeptide-modified gold nanocluster targeted for labeling cell nucleoli and a preparation method thereof, to reduce the background interference of cell imaging of existing cell nucleolus labeling materials and to solve the problem of existing cell nucleoli The problems of high production cost, high toxicity and complicated production process of marking materials

Method used

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  • KCK polypeptide modified gold nanocluster and preparation method thereof
  • KCK polypeptide modified gold nanocluster and preparation method thereof
  • KCK polypeptide modified gold nanocluster and preparation method thereof

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Embodiment 1

[0022] The preparation method of the gold nanocluster modified by the KCK polypeptide involved in this embodiment, the specific process steps are as follows:

[0023] (1) Prepare 20mmol / L HAuCl with ultrapure water 4 , 1.5mol / L NaOH, 20mmol / L tris(2-methylethyl)phosphine hydrochloride (TCEP), 0.1mmol / L NaBH 4 and 20mmol / L KCK solution, soak the glass bottle with aqua regia, clean it and dry it for later use;

[0024] (2) Add 500 μL of KCK solution and TCEP solution to the treated glass bottle, mix well, place in a constant temperature water bath at 70°C and heat for 10 minutes, then add 500 μL of HAuCl to the glass bottle 4 solution, mixed evenly, put it into a constant temperature water bath at 70°C to react for 15 minutes, then take the glass bottle out of the water bath, add magnets to the bottle, and add NaOH solution and NaBH in turn under stirring at 300rpm 4 solution, it can be observed that the color of the solution in the glass bottle changes from light yellow to br...

Embodiment 2

[0034] The preparation method of the gold nanocluster modified by the KCK polypeptide involved in this embodiment, the specific process steps are as follows:

[0035] (1) Prepare 20mmol / L HAuCl with ultrapure water 4 , 1.5mol / L NaOH, 20mmol / L tris(2-methylethyl)phosphine hydrochloride (TCEP), 0.1mmol / L NaBH 4 and 20mmol / L KCK solution, soak the glass bottle with aqua regia, clean it and dry it for later use;

[0036] (2) Add 500 μL of KCK solution and TCEP solution to the treated glass bottle, mix well, place in a constant temperature water bath at 70°C and heat for 10 minutes, then add 500 μL of HAuCl to the glass bottle 4 solution, mixed evenly, put it into a constant temperature water bath at 70°C to react for 15 minutes, then take the glass bottle out of the water bath, add magnets to the bottle, and add NaOH solution and NaBH in turn under stirring at 300rpm 4 solution, it can be observed that the color of the solution in the glass bottle changes from light yellow to br...

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Abstract

The invention belongs to the technical field of functional nanomaterial, and particularly relates to a KCK polypeptide modified gold nanocluster and a preparation method thereof. A peptide sequence as a surface stabilizer is designed, and a simple green hydrothermal synthesis method is employed to prepare a red fluorescent gold nanocluster with target marking effect on cell nucleolus. The gold nanocluster has particle size in the range of 1.8-2.8nm, and obvious absorption peak in the vicinity of 500nm; when the excitation light of 480nm irradiates on the gold nanocluster, 600-800nm interval has strong fluorescence emission, and the emission peak is 680nm; and the gold nanocluster has fluorescence quantum yield of 12%, and has target marking effect on cell nucleolus. The preparation method is simple, practical, and low in cost; the surface material is stabilized by polypeptide to avoid the agglomeration of nanoparticles; and the gold nanocluster has good biological compatibility, low toxicity, good stability, good emission wavelength, good fluorescence property, and is beneficial to getting better nucleolus imaging effect, and has specific marking on cell nucleolus.

Description

Technical field: [0001] The invention belongs to the technical field of functional biological nanomaterials, and specifically relates to a KCK polypeptide-modified gold nanocluster and a preparation method thereof. By designing a polypeptide sequence as a surface stabilizer, a simple and green hydrothermal synthesis method is used to prepare a gold nanocluster that is effective on cell nucleoli. Red fluorescent gold nanoclusters for target labeling. Background technique: [0002] The nucleolus is an important subnuclear structure in the nucleus, which plays an important role in the growth and proliferation of cells. In addition, the nucleolus is also the site of rRNA transcription, translation and ribosome assembly in the nucleus, so it is also called the "ribosome factory". It has even been reported in the literature that the nucleolus is also the target attack site of some viruses. The nucleolus does not exist all the time. It is formed during the interphase of cell divis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/58C09K11/02B22F9/24B22F1/00B82Y40/00C12Q1/02G01N21/64
CPCG01N21/6402G01N21/6456C09K11/025C09K11/58B82Y40/00B22F9/24B22F1/07
Inventor 王晓娟黄方王雅楠曲剑波何化王生杰
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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