Device and method for improving artificial feeding efficiency of cotton bollworms

A cotton bollworm and artificial feed technology, which is applied in the direction of animal husbandry and the like, can solve the problems of high mortality of newly hatched larvae, many labors, irregular pupation, etc., to prevent cross infection between larvae, low comprehensive cost and high efficiency. Effect

Inactive Publication Date: 2016-10-12
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In 1999, our laboratory improved the feed formula of the cotton bollworm, which greatly increased the survival rate, egg production, egg hatching rate, etc. of the cotton bollworm. Directly connect the newly hatched larvae to the feed in the glass tube, 1-2 heads per tube, pick out the 3rd instar larvae with uniform growth from the tube after 7 days, and transfer them to the tube with new feed to continue to raise; This method requires more labor and higher cost (Liang Gemei et al., 1999)
In 2003, Fan Xianlin and others improved the breeding method of cotton bollworm, and proposed that the newly hatched larvae be picked into 10-hole rearing boxes, one head per hole, and put into artificial feed at one time until pupation; but the shortcoming of this method is that the initial The mortality rate of hatched larvae is high, and the growth and development consistency of larvae is not high, resulting in irregular pupation (Fan Xianlin et al., 2003)
In 2015, Qin Qilian improved the breeding technology of cotton bollworm again (Qin Qilian, 2015), and proposed to raise young larvae in insect tanks, and transfer them to 24-hole insect boxes for rearing after the third instar. However, once there are larvae infected with viruses in the insect tank , probably the whole tank of larvae will be affected

Method used

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  • Device and method for improving artificial feeding efficiency of cotton bollworms
  • Device and method for improving artificial feeding efficiency of cotton bollworms

Examples

Experimental program
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Embodiment 1

[0023] This embodiment is used to illustrate a device for improving the efficiency of artificial breeding of cotton bollworms.

[0024] The main body of the device is a 24-hole plate, and according to the size of the 24-hole plate, a piece of blow-molded paper (purchased in a common stationery store) with the same size as the cover of the 24-hole plate is cut and put into the upper cover.

Embodiment 2

[0026] This example is used to illustrate a method for improving the efficiency of artificial breeding of cotton bollworms.

[0027] Get 1g-2g of artificial feed in the 24-well culture box of the device described in Example 1, connect the newly hatched cotton bollworm larvae in the 24-well plate, connect 3 heads in each hole, cover the lid, and put it after binding with a rubber band. Raise under the condition of temperature 27 ± 1 ℃, relative humidity 70 ± 10%, photoperiod 14L: 10D; every treatment repeats three times ( figure 1 ). A 24-well plate that directly covered the upper cover without blown paper was used as a control.

[0028] During the period, the number of cotton bollworm larvae and developmental ages in the 24-well plate were observed every day, and the SPSS 18.0 software was used to analyze the significance of the difference. The t test was used to compare the difference between adding blown paper and not adding blown paper, and evaluating the effect of using b...

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Abstract

The invention relates to insect breeding, and particularly discloses a device and method for improving artificial feeding efficiency of cotton bollworms. The device mainly comprises a pore plate. Blow molding paper with the same size as an upper cover is added into the upper cover of the pore plate. The method comprises the steps that cotton bollworms are bred through the device to third instar larvae, and third instar larvae growing uniformly are selected and transferred into a bollworm breeding pipe to continue to be bred to pupate. In order to solve the problems that young cotton bollworm larvae are high in escape rate, uneven in development progress, prone to cross infection and the like, newly hatched larvae are bred in the 24-pore plate, the blow molding paper is added for prevent escape, and third instar larvae are transferred into the glass bollworm breeding pipe to be bred. By means of difference between the effect obtained by adding the blow molding paper and the effect obtained by adding no blow molding paper, it is proved that the blow molding paper has the beneficial effect on lowering the larva escape rate and improving development uniformity.

Description

technical field [0001] The present invention relates to insect feeding, in particular to the artificial feeding of cotton bollworms. Background technique [0002] Cotton bollworm is an important pest that damages a variety of crops. People have carried out a lot of research on the ecology, biochemical mechanism, molecular mechanism, and control technology of cotton bollworm. An important guarantee for all kinds of research. [0003] In 1999, our laboratory improved the feed formula of the cotton bollworm, which greatly increased the survival rate, egg production, egg hatching rate, etc. of the cotton bollworm. Directly connect the newly hatched larvae to the feed in the glass tube, 1-2 heads per tube, pick out the 3rd instar larvae with uniform growth from the tube after 7 days, and transfer them to the tube with new feed to continue to raise; This method requires more labor and higher cost (Liang Gemei et al., 1999). In 2003, Fan Xianlin and others improved the breeding ...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 梁革梅张万娜马龙王冰洁魏纪珍赵曼
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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