Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Determination method for isoferulic acid in Rhizoma Cimicifugae

A technology of isoferulic acid and determination method, applied in the field of laboratory testing, can solve the problems of high requirements for operators' operating skills, complex pharmacopoeia operation process, etc., and achieves the effects of good repeatability, consistent accuracy and simple operation.

Inactive Publication Date: 2016-09-21
WUZHOU INST FOR FOOD & DRUG CONTROL
View PDF10 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The operation process of the method in the Pharmacopoeia is complicated and requires high skills of the operator

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Determination method for isoferulic acid in Rhizoma Cimicifugae
  • Determination method for isoferulic acid in Rhizoma Cimicifugae
  • Determination method for isoferulic acid in Rhizoma Cimicifugae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Crush Cimicifuga through a pulverizer and pass through No. 3 sieve, about 0.5g, accurately weighed, mix evenly with 1g of quartz sand, set aside, and transfer to a pre-placed glass fiber filter. Add an appropriate amount of quartz sand to the 10ml extraction pool, shake gently to make it on the same level as the pool mouth, and tighten the upper cover of the extraction pool. After the extraction, the extract was transferred to a 50ml volumetric flask, diluted to the mark with 20% methanol, centrifuged at 15000r / min for 3min, and the supernatant was taken for LC determination.

[0038] The extraction conditions (see table 1) are:

[0039] Table 1

[0040]

best practice

Extraction cell volume (ml)

10

extraction solvent

40% Methanol

pressure (psi)

1500

temperature(℃)

120

Static extraction time (min)

5

Static cycle times

4

Flushing volume (%)

60

Purge time (s)

90

[0041] A...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
volumeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a determination method for isoferulic acid in Rhizoma Cimicifugae. The method comprises the following steps: step 1, crushing Rhizoma Cimicifugae and then extracting the crushed Rhizoma Cimicifugae by using an ASE extraction method so as to obtain extract; step 2, subjecting the extract obtained in the previous step to centrifugation so as to obtain supernatant; and step 3, analyzing the supernatant by using liquid chromatography. The determination method for isoferulic acid in Rhizoma Cimicifugae is simple to operate and high in detection precision.

Description

technical field [0001] The invention relates to the field of laboratory testing, in particular to a method for determining isoferulic acid in Cimicifuga. Background technique [0002] In 2010, the Pharmacopoeia recorded the method for extracting isoferulic acid in Cimicifuga: take about 0.5g of the powder of this product (through the No. 2 sieve), accurately weigh it, put it in a stoppered conical flask, add 25ml of 10% ethanol precisely, and seal it tightly. Stopper, weighed, heated to reflux for 2.5 hours, allowed to cool, weighed again, made up the lost weight with 10% ethanol, shaken well, filtered, and obtained the filtrate. Centrifuge at 15000r / min for 3min, take the supernatant, and enter the LC assay. [0003] The parameters of the liquid chromatography are as follows: octadecylsilane bonded silica gel is used as filler; acetonitrile-0.1% phosphoric acid (13:87) is used as mobile phase; detection wavelength is 316nm. [0004] The operation process of the pharmacopo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 陈学松欧妮陈翠玲钟水桥李仁乔
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com