Mycobacterium tuberculosis pyrazinamide molecule drug sensitivity detection method

Abstract

The invention relates to a mycobacterium tuberculosis pyrazinamide molecule drug sensitivity detection method, which aims at solving the problems to find a drug sensitivity target of mycobacterium pyrazinamide, to provide a pyrazinamide drug-resistant mutant library due to a pyrazinamide drug sensitivity target mutation on the basis, and to perform fast drug sensitivity detection by a molecule drug sensitivity detection technology. The detection method provided by the invention is simple and effective; clinic users can use the pyrazinamide molecule drug sensitivity result judgment to monitor the drug sensitivity of clinic tuberculosis patients according to an experiment method; the drug use is guided; the generation of pyrazinamide-resistant strains is reduced; and a novel and effective method is provided for clinic application and scientific study.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for detecting drug sensitivity of mycobacterium tuberculosis pyrazinamide molecules. Background technique [0002] Tuberculosis is still one of the major infectious diseases threatening human health, and there are millions of new tuberculosis cases worldwide every year. my country has invested a lot of manpower and material resources in the prevention and control of tuberculosis. Although my country widely inoculates BCG and treats tuberculosis patients according to WHO standards, the number of people who die from tuberculosis every year still hovers in the top three of major infectious diseases. In recent years, with the prevalence of drug-resistant tuberculosis and multidrug-resistant tuberculosis strains, the co-infection of tuberculosis and AIDS has increased, and the problem of tuberculosis has become more serious. The rising tuberculosis problem has led developed coun...

AI Technical Summary

Problems solved by technology

Although pncA gene mutations are highly correlated with PZA drug resistance, pncA gene mutations are highly diverse and cannot be improved on the basis of current molecular drug sensitivity experiments such as MTBDRplus (HainLifescience) and GeneXpert (Cepheid), and must rely on DNA sequencing technology or Improving the confidence of sequencing technology to rapidly detect the sensitivity of PZA

Although many molecular drug susceptibility methods, such as PCR single-strand conformation polymorphism (PCR-SSCP), gene chips, etc., have been used to detect pncA mutations in PZA-resistant strains, but these tests are more cumbersome and expensive than pncA sequencing. Molecular detection eliminates the process of culturing strains and can shorten the time of drug susceptibility testing to 1 day, so pncA gene sequencing is the best molecular drug susceptibility testing method for PZA

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