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Method and PCR specificity identifying primers for identifying rhizoma bletillae and adulterants of rhizoma bletillae

A confounding and specific technology, used in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. The effect of facilitating rapid identification

Active Publication Date: 2016-08-31
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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  • Method and PCR specificity identifying primers for identifying rhizoma bletillae and adulterants of rhizoma bletillae
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  • Method and PCR specificity identifying primers for identifying rhizoma bletillae and adulterants of rhizoma bletillae

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Embodiment 1

[0028] 1) Materials, reagents and instruments

[0029] a.Material

[0030] The materials are shown in Table 1 below. Baiji, Bingqiuzi, Jianxueqing, Dusuanlan, Tongbanlan, and Vietnam Baiji are samples to be identified. These materials can be collected or purchased from the market.

[0031] Table 1 Sample source table

[0032]

[0033] b. Reagent agarose (Promega, USA); DL2000 (Beijing Dingguochangsheng Company);

[0034] 10×PCR Taq buffer (Mg 2+ plus 20mM) (Beijing Dingguo Changsheng Company); 10mM dNTP

[0035] (Takara biobao Biological Engineering (Dalian) Co., Ltd.); nucleic acid dye (Beijing Dingguochangsheng Company); primers were synthesized by Shanghai Sangon Biotechnology Co., Ltd.; 2U / μL DNA Taq polymerase (Beijing Dingguochangsheng Company); 10 -100ng DNA template (Beijing Dingguochangsheng Company).

[0036] c. Instrument PCR instrument (Bio-RAD T100 Thermal Cycler); gel imaging system (G-BOX F3 British Syngene); electrophoresis instrument (Beijing Liuyi Ins...

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Abstract

The invention discloses a method and PCR specificity identifying primers for identifying rhizoma bletillae and adulterants of rhizoma bletillae. According to the specificity identifying primers designed through the specific site PCR technology, the base sequence of the upstream primer BJ-F is shown as SEQ ID NO:1, the base sequence of the upstream primer BJ-R is shown as SEQ ID NO:2, and the length of a target product segment is 508 bp. By means of a PCR amplification reaction system and reaction conditions determined through the primers and the method, true rhizoma bletillae and counterfeit drugs can be identified within a week with 1 g of sample. The method and the primers have the advantages of being easy to operate, quick, accurate and small in sample use quantity, have a unique advantage in the aspect of medicine true and counterfeit drug quick detection, and are beneficial to quick identification of rhizoma bletillae and other traditional Chinese medicine.

Description

technical field [0001] The invention relates to the technical field of molecular markers, in particular to a method for a set of specific PCR identification primers and their white and confounding products. Background technique [0002] Bletilla Rchb.F is a perennial herbaceous plant in the family Orchidaceae. It is not only of high ornamental value, but also one of the commonly used traditional Chinese medicines. The name of the medicine is Baiji, and the pseudobulbs are used for medicinal purposes. It has the effects of astringent and hemostasis, clearing away heat and detoxification, reducing swelling and promoting muscle growth. There are about 6 species of white and genus in the world, which are distributed in the north of Burma in Asia, through China to Japan. There are 4 kinds of Chinese white ji (Bletilla sinensis), small bletilla striata (Bletilla formosana), white ji (Bletilla striata) and yellow-flowered bletilla striata (Bletilla ochracea). Distributed in Jiang...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 季鹏章杨雁朱新焰李彦莹狐小斌董志渊金航杨天梅张智慧王家金
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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