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Traditional Chinese medicine Chinese soapberry fruit extract for treating diarrhea of yak calves

A kind of Sapinberry extract and technology of Sapinberry, which is applied in the fields of veterinary medicine and animal husbandry, can solve problems such as the application of Sapinberry and marigold that have not yet been seen, and achieve the effect of treating diarrhea in calves and yaks, and the preparation method is simple

Inactive Publication Date: 2016-08-31
林天样
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no application of Sapindus and Marigold in the treatment of calf diarrhea

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: the preparation of Sapindus chinensis extract

[0017] (a) Mix 5 kg of dried Sapindus chinensis and marigold (accounting for 10% of the total weight), pulverize, extract with 90% ethanol solution under heat reflux (20 L × 3 times), combine the extracts, concentrate until there is no alcohol smell to obtain ethanol Extract the concentrated solution; (b) dilute the ethanol extracted concentrated solution obtained in step (a) with water (3L), and successively use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol Extract (3L×3 times), concentrate under reduced pressure to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) n-butanol extract is dissolved in water, filtered, and filtered with AB-8 To enrich the active ingredients in the resin, first wash 10 column volumes with 15% ethanol to remove large polar components, then elute with 90% ethanol for 15 column volumes, collect the ...

Embodiment 2

[0018] Embodiment 2: the preparation of Sapinberry extract

[0019] (a) Mix 5 kg of dried Sapindus chinensis and marigold (5% of the total weight), pulverize, extract under reflux with 85% ethanol solution (20 L × 3 times), combine the extracts, concentrate until there is no alcohol smell to obtain ethanol Extract the concentrated solution; (b) dilute the ethanol extracted concentrated solution obtained in step (a) with water (3L), and successively use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol Extract (3L×3 times), concentrate under reduced pressure to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) n-butanol extract is dissolved in water, filtered, and filtered with AB-8 To enrich the active ingredients in the resin, wash 12 column volumes with 10% ethanol to remove large polar components, then elute 16 column volumes with 85% ethanol, collect the 85% ethanol eluate, and concentrate und...

Embodiment 3

[0020] Embodiment 3: the preparation of the Sapindus chinensis extract

[0021] (a) Mix 5 kg of dry Sapindus chinensis and marigold (accounting for 15% of the total weight), pulverize, extract with 95% ethanol solution under heat reflux, combine the extracts (20L × 3 times), concentrate until there is no alcohol smell to obtain ethanol Extract the concentrated solution; (b) dilute the ethanol extracted concentrated solution obtained in step (a) with water (3L), and successively use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) extraction, concentration under reduced pressure to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; To enrich the active ingredients in the resin, wash 8 column volumes with 20% ethanol to remove large polar components, then elute 14 column volumes with 95% ethanol, collect the 95% ethanol eluate, and concentrate under reduced pressure.

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PUM

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Abstract

The invention discloses a traditional Chinese medicine Chinese soapberry fruit extract for treating diarrhea of yak calves. A method for preparing the Chinese soapberry fruit extract comprises the following steps: (a) mixing dried Chinese soapberry fruits with marigolds, crushing the mixture, carrying out heat reflux extraction by using a 85-95% alcohol solution, merging the extracting solution, and carrying out concentrating until the mixed solution is free from alcohol taste to obtain an alcohol extraction concentrated solution; (b) diluting the alcohol extraction concentrated solution obtained in the step (a) with water, sequentially extracting the obtained diluent by using petroleum ether, ethyl acetate and water-saturated n-butyl alcohol, and carrying out vacuum concentration to respectively obtain petroleum ether extract, ethyl acetate extract and n-butyl alcohol extract; and (c) dissolving the n-butyl alcohol extract with water, carrying out filtering, enriching active ingredients by using AB-8 type macroporous resin, firstly using 10-20% alcohol to wash 8-12 column volume so as to remove high-polarity ingredients, then using 85-95% alcohol to elute 14-16 column volume, collecting the eluant of the 85-95% alcohol, and carrying out vacuum concentration, wherein the weight of the marigolds accounts for 5-15% of the total weight of the Chinese soapberry fruits and marigolds.

Description

technical field [0001] The invention belongs to animal husbandry, and relates to veterinary medicine, in particular to an extract of Sapindus japonica, a traditional Chinese medicine for treating calf yak diarrhea. The extract has obvious therapeutic effect on calf yak diarrhea. Background technique [0002] Calf diarrhea is a common disease in pastoral areas, with high morbidity and mortality, which seriously threatens the development and production efficiency of animal husbandry. The etiology of diarrhea is complex and diverse. Factors such as bacteria, viruses, parasites, indigestion and environment can cause diarrhea. Among them, enteropathogenic Escherichia coli and Salmonella are the main pathogens that cause diarrhea in calf yaks. [0003] Sapindus is the seed of Sapindaceae plant Sapindus. Ripe fruit is picked, the pulp is removed, and the seeds are dried in the sun. The original plant is a deciduous or evergreen tree, up to 25 meters. Branches spreading, branchle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/77A61P1/12A61K36/28
CPCA61K36/77A61K36/28A61K2236/333A61K2236/51A61K2236/53A61K2236/55A61K2300/00
Inventor 林天样
Owner 林天样
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