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A kind of rapid somatic embryo seedling breeding method of Zhejiang safflower camellia oleifera

A technology of safflower camellia and rapid breeding seedlings, which is applied in the field of plant tissue culture, can solve the problems that Zhejiang safflower camellia tissue culture technology has not been reported, and the natural reproduction rate of camellia oleifera is low, and achieve good practical effect

Active Publication Date: 2018-03-13
浙江萧建集团有限公司
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Problems solved by technology

[0003] The natural reproduction rate of camellia oleifera is low, so tissue culture rapid propagation technology can be used to solve the problem. At present, there are no reports on tissue culture technology related to Zhejiang safflower camellia oleifera at home and abroad.

Method used

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  • A kind of rapid somatic embryo seedling breeding method of Zhejiang safflower camellia oleifera
  • A kind of rapid somatic embryo seedling breeding method of Zhejiang safflower camellia oleifera
  • A kind of rapid somatic embryo seedling breeding method of Zhejiang safflower camellia oleifera

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Embodiment 1

[0028] A kind of somatic embryo rapid breeding seedling method of Zhejiang safflower camellia, the steps are as follows:

[0029] 1) Pretreatment of explants

[0030] The seeds of Zhejiang Honghua Camellia oleifera harvested in October 2014 in Wuyi Mountain, Fujian Province were selected as materials, and the seed shells were carefully removed to avoid damage to the seeds. Soak the seeds in water for 1.5 hours. Afterwards, the seed coat is removed. Wash with detergent and rinse with running water for 2 hours.

[0031] 2) Sterilization of safflower camellia fruit and establishment of aseptic system

[0032] Wipe the ultra-clean workbench with 75% alcohol, and turn on the ultraviolet lamp to sterilize for 20 minutes. Put the seeds rinsed in running water into a sterile bottle with tweezers, pour 75% alcohol, completely immerse the seeds in the alcohol, stir and sterilize for 30 seconds, pour out the alcohol and rinse once with sterile water. Pour in 0.1% mercury liter and s...

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Abstract

The invention discloses a somatic embryo rapid propagation and seedling growing method for camellia chekiangoleosa. The method comprises the steps of 1, pretreatment of explants; 2, sterilization of camellia chekiangoleosa fruits and establishment of a sterile system; 3, inoculation and induction of calluses; 4, induction of somatic embryos; 5, induction of secondary embryos; 6, differentiation of the secondary embryos into strong seedlings. According to the somatic embryo rapid propagation and seedling growing method for camellia chekiangoleosa, N6+NAA 1.0 mg / L+6-BA 1.0 mg / L+2,4-D 1.0 mg / L is used as a medium for inducing the embryonic calluses; after inoculation, cotyledon pieces on the medium begin to expand after about 7 d of culture, and a large quantity of faint yellow and dense embryonic calluses with smooth and lustrous surfaces begin to appear around the cotyledon pieces after about 15 d; after the embryonic calluses are transferred to a somatic embryo induction medium, the somatic embryos begin to appear after about 20 d; through proliferation and differentiation culture of the secondary embryos, sprouts and roots appear through differentiation after about 15 d, and finally the strong seedlings are formed. An efficient camellia chekiangoleosa somatic embryogenesis system is established through the method, and a large quantity of seedlings is provided for camellia chekiangoleosa.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rapidly growing somatic embryo seedlings of Zhejiang safflower camellia oleifera. Background technique [0002] Zhejiang safflower camellia, alias: Zhejiang red camellia. scientific name: Camellia chekiangoleosa . Camellia, the genus Camellia, is a unique tree species in my country. Distributed in southern Zhejiang, eastern Jiangxi, and high mountains on the borders of Zhejiang, Fujian, and Anhui provinces. Zhejiang safflower camellia has two uses: ornamental and oil. The plant has beautiful shape, emerald green leaves, beautiful and huge safflowers, bright colors, fruit shaped like apples, full of red fruits in summer and autumn, strong cold resistance, and is an excellent parent for camellia resistance breeding. This tree species has a high yield of tea seed oil and good oil quality, and the quality and yield of camellia oil are better...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 续晨温强徐雯蔡小宁贲爱玲杨平徐立军赵俊丽段淑蓉华春
Owner 浙江萧建集团有限公司
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