Method for producing chondroitin sulfate

A chondroitin sulfate and production process technology, applied in the field of bioengineering, can solve problems such as waste of raw materials, waste, complex extraction process, etc., and achieve the effects of reducing BOD and COD, reducing industrial energy consumption, and avoiding environmental pollution

Inactive Publication Date: 2016-08-24
管桂义
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional extraction method of chondroitin sulfate in animal cartilage tissue is time-consuming and complicated, and the purity of chondroitin sulfate is low, which is not conducive to the industrial production of chondroitin sulfate; at the same time, a large amount of raw materials are wasted in the production process of chondroitin sulfate waste and pollute the environment

Method used

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Experimental program
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Embodiment 1

[0021] A method for producing chondroitin sulfate from animals, comprising the steps of:

[0022] 1) Raw material handling

[0023] The nasal bone, throat bone, soft rib, trachea, crescent bone, and miscellaneous cartilages of various parts of animals that have passed the quarantine, after removing fat and connective tissue, air-dried naturally, put them in a pulverizer, and passed through a sieve of no less than 80 meshes to obtain cartilage powder spare;

[0024] 2) hot alkali dissolution

[0025] Add the cartilage powder into a reaction tank with a mass percentage concentration of 2% to 6% NaOH solution, the ratio of material to liquid is 1:4, heat to 80-97°C, stir evenly and keep it for 10-30 minutes;

[0026] 3) Acid regulating enzymatic hydrolysis

[0027] Pump the feed liquid into another reaction tank, add hydrochloric acid solution with a concentration of 10-20% by volume, adjust the pH of the extraction solution to 5-8, and the temperature is 40-65°C, add papain, ...

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Abstract

The invention belongs to the bioengineering field, and discloses a method for producing animal chondroitin sulfate. An animal by-product, namely cartilage, is used as a raw material; the method comprises the following steps of: performing hot alkali dissolution; performing ultrasonic extraction to improve the extraction efficiency; regulating acid, performing double enzymolysis by adopting plant protease and microbial protease to reduce the production cost while improving the yield of a product; removing impurities and decoloring, and performing further precipitation by using ethanol to improve the purity of the product; and finally, performing centrifugal drying, crushing, and packing to obtain the chondroitin sulfate finished product. The chondroitin sulfate produced by the production process provided by the invention has the characteristics of high extraction efficiency, stable quality, short production time, and change from waste to treasure; at the same time, a nutritious feed is prepared, no any waste is produced, environmental pollution is avoided, industrial energy consumption is reduced, and the chondroitin sulfate can be applied to large-scale industrialized production.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for producing chondroitin from animals. Background technique [0002] Chondroitin sulfate (Chs for short) is an acidic mucopolysaccharide extracted from cartilage such as mammalian trachea. White powder, odorless, tasteless, easy to absorb moisture, soluble in water, insoluble in organic solvents such as ethanol and acetone, it swells or becomes viscous when it meets water, and is relatively unstable to heat. It needs to be stored away from light and sealed. Chondroitin is a mucopolysaccharide composed of D-glucuronic acid and N-acetyl-D-galactosamine. Chondroitin sulfate is the sulfate ester of chondroitin, a major component of connective tissue. It has the functions of clarifying lipids, improving the detoxification function of the body, diuresis and analgesia. It is very effective for collagen diseases and also effective for hearing impairment caused by s...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 管桂义
Owner 管桂义
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