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Application of deuterium-depleted water as tumor multidrug-resistance reversal agent

A multi-drug resistance, deuterium-depleted water technology, applied in anti-tumor drugs, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of increasing tumor cell drug sensitivity and multi-drug resistance, etc. Overcoming multidrug resistance and reducing toxicity

Inactive Publication Date: 2016-08-24
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of deuterium-depleted water as an adjuvant therapy for tumor therapy to reverse tumor multidrug resistance and increase tumor cell drug sensitivity.

Method used

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  • Application of deuterium-depleted water as tumor multidrug-resistance reversal agent
  • Application of deuterium-depleted water as tumor multidrug-resistance reversal agent
  • Application of deuterium-depleted water as tumor multidrug-resistance reversal agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The inhibitory effects of deuterium-depleted water and cisplatin on lung cancer A549 cells and lung cancer multidrug-resistant A549 / DDP cells were detected by MTT method. Lung cancer A549 cells and lung cancer multidrug-resistant A549 / DDP cells were starved for 12 hours in serum-free 1640 medium, digested with EDTA-containing trypsin, and divided into 5×10 3 Cells / well were seeded in 96-well plates. Add 100 μl of common 1640 medium containing cisplatin at concentrations of 0, 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 μg / ml to the 96-well plate with lung cancer A549 cells, and then add lung cancer multidrug-resistant 0, 2, 4, 6, 8, 10, 12, 14, and 16 μg / ml of deuterium-depleted water medium (50 ppm) were added to the 96-well plate of DDP cells, respectively. After culturing for 24 hours, 20 μl of MTT solution was added to each well to continue culturing for 4b, the culture solution was aspirated, and 150 μl of dimethyl sulfoxide (DMSO) was added to each well, and shaken for 10 m...

Embodiment 2

[0031] Effect of deuterium-depleted water and cisplatin on the colony formation ability of lung cancer A549 cells and lung cancer multidrug-resistant A549 / DDP cells by plate cloning test. Lung cancer A549 cells and lung cancer multidrug-resistant A549 / DDP cells were starved for 12 hours in serum-free 1640 medium, digested with EDTA-containing trypsin, and seeded in 6-well plates at a density of 500 cells / well. Correspondingly, culture media with deuterium concentrations of 150ppm, 75ppm, and 50ppm were added to each well of the orifice plate, and the concentration of cisplatin in the culture medium was 1 μg / ml. After 2-3 weeks, when the cell clone colonies in the well plate are almost fused, the culture is terminated. Aspirate the culture medium and wash carefully 2-3 times with PBS. Add 3ml of 4% paraformaldehyde to each well to fix for 15min; absorb the paraformaldehyde, add crystal violet staining solution to each well for staining for 20min, and recover crystal violet. R...

Embodiment 3

[0033] The effects of deuterium-depleted water and cisplatin on the cell cycle of lung cancer multidrug-resistant A549 / DDP cells were detected by flow cytometry. The lung cancer multidrug-resistant A549 / DDP cells were starved for 12 hours in serum-free 1640 medium, digested with EDTA-containing trypsin, and diluted with 1×10 4 Cells / well were seeded in 6 cm culture dishes. Add deuterium concentrations of 150ppm, 100ppm, 75ppm, and 50ppm to culture medium containing cisplatin at a concentration of 1 μg / ml and culture for 24 hours, then suck off the medium, wash with PBS for 2-3 times, digest with EDTA-free trypsin and collect cell. Centrifuge at 1500rpm / min for 3min, discard supernatant; resuspend in PBS, centrifuge at 1500rpm / min for 3min; discard supernatant; repeat resuspension, centrifugation, discard supernatant; add 1ml of 70% ethanol, fix overnight at 4°C. Centrifuge at 1500rpm / min for 3min, discard the supernatant; repeat resuspension, centrifugation, and discard the ...

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Abstract

The invention belongs to the field of medicine, and relates to an application of deuterium-depleted water (DDW) in reversing tumor multidrug resistance and an application as an antineoplastic drug sensitizer. The deuterium-depleted water, which has an effect of reversing the multidrug resistance of tumor cells as a natural product, can be used as a tumor multidrug-resistance reversal agent; and meanwhile, the deuterium-depleted water, which has an effect of enhancing the sensitivity of tumor multidrug-resistance cells to an antineoplastic drug, can be used as the antineoplastic drug sensitizer. The deuterium-depleted water provided by the invention, as ab adjuvant therapy agent for tumor therapy, can be independently used or used in a mode of combining with one or more antineoplastic drugs selectively. The invention also relates to independent use of the deuterium-depleted water or the combined use of the deuterium-depleted water with one or more antineoplastic drugs in daily tumor therapy, wherein the deuterium content in the deuterium-depleted water is 0.01-100ppm.

Description

technical field [0001] The invention belongs to the medical field of reversing tumor multidrug resistance and increasing the sensitivity of tumor cells to antitumor drugs, and in particular relates to the role of deuterium depleted water (DDW) in reversing tumor multidrug resistance and antitumor drug proliferation. application of sensitizers. Background technique [0002] Malignant tumor (cancer) is one of the main causes of human death. According to the report of the World Health Organization, more than 7 million people die of tumor every year, of which 60% die from lung cancer, gastric cancer, breast cancer, colorectal cancer, oral cancer, Liver cancer, cervical cancer, and esophageal cancer account for about 13% of all deaths. As the world population ages, the number of cancer deaths worldwide is projected to continue to rise and may exceed 13.1 million by 2030. Chemotherapy is currently one of the main means of clinical treatment of malignant tumors, and the generatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/00A61P35/00
Inventor 杨慧龄张瀚彬祝葆华王宏强陈楚言
Owner GUANGDONG MEDICAL UNIV
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