Nematicidal-activity P. oxalicum NBC012 and preparation method and application thereof

A nematicidal and active technology is applied in the field of fungi capable of killing plant-parasitic nematodes and the preparation thereof, and can solve the problems of less nematodes and the like

Inactive Publication Date: 2016-08-17
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, few biocontrol agents have been developed to control nematodes

Method used

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  • Nematicidal-activity P. oxalicum NBC012 and preparation method and application thereof
  • Nematicidal-activity P. oxalicum NBC012 and preparation method and application thereof
  • Nematicidal-activity P. oxalicum NBC012 and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Preparation of bacterial strain P.oxalicum NBC012 fermented liquid

[0034]Inoculate the P. oxalicum NBC012 mycelia on a PDA plate, and cultivate it at 26°C for 10 days. Use a sterilized hole puncher to inoculate a bacterium cake with a diameter of 1 cm from the plate into a 250ml Erlenmeyer flask (with 100ml PDB inside), 26 Cultivate with shaking at 150rpm / min in the dark for 120h. Use 4 layers of sterilized gauze to remove the mycelia and pass through a 0.22 μm bacterial filter to sterilize to obtain the fermentation stock solution. The fermentation stock solution was diluted in different times to determine the nematicidal efficacy.

Embodiment 2

[0035] Example 2: Nematicidal activity of strain P. oxalicum NBC012

[0036] On the basis of the stock solution of the fermentation broth, it was diluted 1×, 5× and 10× sequentially, and the 2nd instar larvae of soybean cyst nematode were tested.

[0037] 1 Preparation of test agents

[0038] The fermented liquid of the fungal strain P.oxalicum NBC012 prepared according to the aforementioned liquid fermentation method was used to measure the nematicidal activity of the fermented liquid with different dilution ratios.

[0039] 2 Preparation of nematodes for experiment

[0040] 2.1 Collection of soybean cyst nematode cysts

[0041] Pour the diseased soil harmed by soybean cyst nematodes collected from the Langfang pilot test base of the Academy of Agricultural Sciences into a clean plastic small bucket, wash away the diseased soil with strong water, pass through a 20-mesh / 80-mesh sieve, and collect 80-mesh Sediments on the mesh screen, cysts were picked under a dissecting mic...

Embodiment 3

[0066] Example 3: Determination of indoor control effect of bacterial strain P.oxalicum NBC012 fermentation broth

[0067] Using fermented stock solution to control soybean cyst nematode and wheat cyst nematode, the effect is as follows:

[0068] 1 Test materials:

[0069] Soybean variety: Zhonghuang 13

[0070] Nematode: soybean cyst nematode

[0071] 2 Test method:

[0072] Determination of the effect of fermentation broth of strain P.oxalicum NBC012 on indoor control of soybean cyst nematode

[0073] The surface of soybean seeds was sterilized with 1% sodium hypochlorite, washed three times with sterilized water, and placed in a disposable petri dish to germinate. After 2 days the germinated seeds were planted in plastic cups filled with sterilized soil, 3 seeds per cup. One week later, 20 ml of the fungal fermentation stock solution was added to the plastic cup, and PDB was used as a control. After 24 hours of fermentation, inoculate 300 SCN second-instar larvae near...

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Abstract

The invention relates to nematicidal-activity P. oxalicum NBC012 and a preparation method and application thereof. A high-activity fungus strain P. oxalicum NBC012 is isolated and screened from wheat cyst nematode group from stfold, Norway and is collected under CGMCC No. 12474. Nematicidal-activity P. oxalicum NBC012 is prepared by liquid fermentation culturing, fermentation broth of P. oxalicum NBC012 has significant poisoning action for plant parasitic nematodes. Indoor determination shows that the fermentation broth of the strain has effect on age-2 larvae and egg hatching of soybean cyst nematode. Fermentation broths with different dilution concentrations all show poisoning action for age-2 larvae and significant inhibitory action for egg hatching. Fermentation original broth may cause a decrease of 57.45% in amount of soybean cyst, and P. oxalicum NBC012 has a promising development and application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, in particular to a fungus capable of poisoning and killing plant parasitic nematodes and its preparation method and application. Background technique [0002] Plant parasitic nematodes are one of the important plant pathogens, which generally occur all over the world, have a wide range of hosts, and cause serious damage. The annual economic losses caused by plant parasitic nematodes in the world are as high as 157 billion U.S. dollars (Jian Heng, 2011. Plant Nematology . Beijing: China Agricultural University Press). Among them, soybean cyst nematode (Soybean Cyst Nematode, SCN) is one of the most serious plant parasitic nematodes. [0003] Soybean cyst nematode was first discovered in my country (1899), and its original scientific name was Heterodera schachtii, which is the scientific name of sugar beet cyst nematode. In 1952, through careful observation, Ichinohe independently es...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/80
CPCA01N63/30C12N1/14C12N1/145C12R2001/80
Inventor 彭德良李婷黄文坤孔令安彭焕崔江宽
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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