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A method for improving the biomass of lactic acid bacteria under high-salt conditions

A technology of lactic acid bacteria and biomass, which is applied in the field of fermentation engineering, can solve the problems of hindering the density of bacteria and the difficulty of high-density cultivation of lactic acid bacteria in high-activity lactic acid bacteria starter, and achieves the ability to resist high-salt stress, good growth performance, and simple method easy effect

Active Publication Date: 2019-06-25
SICHUAN UNIV
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Problems solved by technology

[0003] Salt stress also hinders the further increase of cell density, making it more difficult to prepare highly active lactic acid bacteria starter and high-density culture of lactic acid bacteria

Method used

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  • A method for improving the biomass of lactic acid bacteria under high-salt conditions

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Experimental program
Comparison scheme
Effect test

example 1

[0011] Example 1: When the NaCl concentration in the fermentation medium is 12%, the growth of T.halophilusCGMCC NO.3792 (Table 1)

[0012] The seed solution was inoculated into the fermentation medium with 0.5g / L proline (addition group) and the fermentation medium without proline (control group) with 5% inoculation amount, and the NaCl concentration was adjusted to 12%. , pH 7.0. After 48h of fermentation, measure the OD 600 The OD value of the sample added with proline was 34.7% higher than that of the sample without proline added.

example 2

[0013] Example 2: When the NaCl concentration in the fermentation medium is 18%, the growth of T.halophilusCGMCC NO.3792 (Table 1)

[0014] Take the seed liquid and inoculate it into the fermentation medium with 0.5g / L proline (addition group) and the fermentation medium without proline (control group) at an inoculation amount of 5%, and adjust the NaCl concentration to 18%. , pH 7.0. After 48h of fermentation, measure the OD 600 The OD value of the sample added with proline was 30.6% higher than that of the sample without added proline.

[0015] Table 1 Bacterial biomass in high-salt fermentation medium

[0016]

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Abstract

The invention discloses a method for improving biomass of lactic acid bacteria under the high salt condition and belongs to the technical field of bioengineering. Proline improves biomass of lactic acid bacteria in a high salt fermentation medium. The method has simple processes, is convenient for operation, effectively improves growth performances of lactic acid bacteria in a high salt environment and provides a referential method for an industrial biotechnology and especially for high density culture of lactic acid bacteria under salt stress.

Description

technical field [0001] The invention relates to a method for increasing the biomass of lactic acid bacteria under high-salt conditions by adding proline, and belongs to the technical field of fermentation engineering. Background technique [0002] Small molecule organic acids such as lactic acid, the metabolite of lactic acid bacteria, can not only produce ester components through condensation reaction with alcohols, endow the product with unique flavor, but also reduce the pH of the system and inhibit harmful microorganisms, such as Escherichia coli, Bacillus subtilis, etc. growth. Therefore, lactic acid bacteria have been widely used in the production of high-salt fermented condiments (soy sauce, fish sauce, soybean paste). In the production of different types of fermented condiments, the salt content is quite different, and the fluctuation range is also different. The high-salt stress environment seriously affects the normal physiological metabolism of lactic acid bacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12R1/01
Inventor 吴重德何桂强黄钧周荣清
Owner SICHUAN UNIV
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