Establishing method for in-vitro regeneration system of Kiwi berry

An in vitro regeneration and kiwifruit technology, applied in the field of plant plant tissue culture, can solve the problems of grafting propagation and cutting propagation that cannot be propagated quickly, in large quantities, and with high quality, and achieves the effect of meeting market demand.

Inactive Publication Date: 2016-08-03
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for establishing the in vitro regeneration system of Actinidia jujube suitable for North China and the Yangtze River Basin, aiming to solve the problem that traditional grafting and cutting propagation cannot be reproduced quickly, in large quantities, and with high quality, and because of different kiwifruits and different varieties. Problems requiring selection of suitable media due to genotype differences

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  • Establishing method for in-vitro regeneration system of Kiwi berry
  • Establishing method for in-vitro regeneration system of Kiwi berry
  • Establishing method for in-vitro regeneration system of Kiwi berry

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[0029] In order to make the purpose and technical solution of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only for explaining the present invention, and are not intended to limit the present invention.

[0030] The application principles of the present invention will be further described below according to specific embodiments.

[0031] The present invention adopts the new slightly stem section, blade and petiole of Actinidia jujuba that just germinated in spring as the explant material, preferably adopts the new slightly stem section, blade and petiole of the all-red type Actinidia jujube 'Tianyuanhong' that just germinated in spring as the explant material. Explant material. The following will take the whole red type kiwifruit 'Tianyuanhong' as an example to further illustrate the present invention. It should be noted ...

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Abstract

The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for establishing a kiwifruit in vitro regeneration system, which comprises: the steps of selecting and sterilizing explants, the step of inducing adventitious buds, the step of multiplying adventitious buds, the step of inducing rooting, and Seedling hardening and transplanting steps. According to the method for establishing the kiwifruit in vitro regeneration system provided by the present invention, the axillary bud induction rate reaches 100%, the leaf callus induction rate reaches 92%, the germination rate is 40%, the adventitious bud proliferation coefficient is 4.5, and the rooting rate can reach 96%. Not only can the kiwifruit reproduce rapidly, in large quantities and with high quality, but also the leaf callus induction step can provide a basis for the study of kiwifruit genetic transformation.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for establishing a kiwifruit in vitro regeneration system. Background technique [0002] Actinidiaaguta, also known as soft jujube, kiwi pear, and vine melon, belongs to Actinidiaceae (Actirlidiaceae) genus Actinidia Lindl. It is a perennial woody vine, distributed in northeast, north, northwest and the provinces of the Yangtze River Basin in my country . Jujube kiwifruit is rich in various nutrients and has medical and health care effects. Jujube kiwifruit is rich in vitamin C, up to 430.8mg / 100g, contains nearly 20 kinds of amino acids, the sum of various amino acids is 12155.89mg / 100g, and more than 20 kinds Inorganic elements; kiwifruit is also rich in superoxide dismutase (SOD), which has the functions of improving immune function, anti-cancer, anti-aging, softening blood vessels, etc. At the same time, compared with Chinese kiwifruit and de...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 林苗苗方金豹齐秀娟陈锦永顾红张威远张洋孙雷明
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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