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Mycoplasma pneumoniae detection kit

A technology of Mycoplasma pneumoniae and detection kit, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of detection of Mycoplasma pneumoniae IgM antibody that have not been reported, and achieve convenient clinical detection, reduce inter-batch and intra-batch differences, and coincidence rate high effect

Inactive Publication Date: 2016-07-13
山东德诺生物科技有限公司
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AI Technical Summary

Benefits of technology

This patented describes an assay system called Methylene blue Immunofluor® Quick Test Kit used to detect mycotic bronchitis virus type A(MyCOV). These tests are quicker but more reliable compared to existing methods such as Western Blot analysis. They also provide improved reproducibility due to their ability to capture multiple strains from different sources during experimentation. Additionally, they allow for direct comparison among various samples taken together while maintaining consistent quality control standards. Overall, these technical feats improve the speed and precision of diagnosing Brucella' disease caused by Bordetella pertinii.

Problems solved by technology

Technologies described involve various techniques including culturing cells called MPC(mycrozoa pathogens) isolated from individuals who develop pulmonitis due to their exposure to this agent. These tests provide quick results without requiring expensive facilities. They work well even during acute illness outbreaks where they could cause complications like sepsis. To overcome these limitations, research efforts aim towards developing faster and more sensitive ways to identify MPN inhalant pleuropeldnai meningoitrilean paraphylaxis syndrome (MPNS) through specific binding reactions between proteins found within them.

Method used

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Embodiment Construction

[0023] The preparation of embodiment detection test paper

[0024] 1. Materials:

[0025] Fluorescein Alexa 647: Invitrogen

[0026] P1 Recombinant Antigen: Shenzhen Faipeng Biological Co., Ltd.

[0027] MP native antigen: Microbix

[0028] Mouse anti-human IgM μ chain monoclonal antibody: Shenzhen Faipeng Biological Co., Ltd.

[0029] Rabbit IgG antibody and goat anti-rabbit IgG antibody: Sigma

[0030] Sucrose, PVP and Tween-20, etc.: Sinopharm Chemical Reagent Co., Ltd.

[0031] Mycoplasma pneumoniae IgM colloidal gold detection kit: Weifang Kanghua Biotechnology Co., Ltd.

[0032] EU Mycoplasma pneumoniae IgMELISA detection kit: EUROIMMUNUS company

[0033] Influenza A virus, influenza B virus, respiratory syncytial virus, adenovirus, Chlamydia pneumoniae, Q fever Rickettsia, parainfluenza virus, Legionella pneumophila and other respiratory pathogen positive serum, MP positive serum and MP negative serum: Shenyang CDC, PLA 304 Hospital and PLA 307 Hospital.

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Abstract

The invention discloses a kit and detection method for mycoplasma pneumoniae IgM on the basis of a fluorescence immunoassay chromatographic technique.The method comprises the steps that fluorescein serves as a marking material, and a mouse antihuman IgM mu chain monoclonal antibody is marked.A mycoplasma pneumoniae specific IgM antibody in serum is combined with a fluorescein marked antibody to form a reaction compound which is caught by a mycoplasma pneumoniae antigen, the mycoplasma pneumoniae IgM catch yield is in positive correlation with signal strength of the fluorescent antibody, and the mycoplasma pneumoniae IgM in the serum can be detected out by means of an immunofluorescence detector reading.The method has the advantages of being short in time, good in specificity, good in accuracy and stability and the like, and the coincidence rate of the detection result with that of a European Union mycoplasma pneumoniae IgM detection kit is high.A new method for rapidly and accurately detecting the mycoplasma pneumoniae IgM in clinic is provided, and a good market prospect is achieved.

Description

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Claims

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Application Information

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Owner 山东德诺生物科技有限公司
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