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A kind of anti-RSV virus Chinese medicine compound extract and its preparation method and application

A technology of extract and dandelion extract, applied in the field of traditional Chinese medicine extraction and application, can solve the problems of unclear mechanism of action, difficulty in popularization and application, and lack of therapeutic effect of antibodies

Inactive Publication Date: 2019-08-27
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no effective treatment drug specifically for RSV infection. Although the broad-spectrum antiviral drug ribavirin is the only small molecule chemical drug that can be applied to RSV, due to the existence of unknown mechanism of action, insignificant efficacy and toxic side effects, etc. The problem is that its clinical application is very limited, and the antibody drug RSV neutralizing antibody has no therapeutic effect and can only be used for prevention, which is difficult to popularize
[0005] With the deepening of research and the advancement of technology, there are currently a small number of candidate drugs specifically targeting RSV infection in clinical trials, including the small molecule drug GS-5806, which inhibits the fusion process of the RSV envelope with the host cell membrane, and the siRNA drug ALN. - RSV01, nucleoside inhibitor ALS-8176 and N protein inhibitor RSV604, etc., but the outlook is not clear

Method used

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  • A kind of anti-RSV virus Chinese medicine compound extract and its preparation method and application
  • A kind of anti-RSV virus Chinese medicine compound extract and its preparation method and application
  • A kind of anti-RSV virus Chinese medicine compound extract and its preparation method and application

Examples

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Effect test

preparation example 1

[0092] Preparation Example 1: Preparation of Honeysuckle Extract

[0093] A1: Fully pulverize the honeysuckle, pass through a 100-mesh sieve, and obtain the honeysuckle powder;

[0094] A2: Add honeysuckle powder to 10 times the weight of deionized water, stir and extract at 85°C for 45 minutes, filter to obtain the extract;

[0095] A3: Concentrate the extract to 1 / 8 of the original volume to obtain a concentrated solution; add an equal volume of petroleum ether to the concentrated solution, fully oscillate for extraction, and separate the petroleum ether phase and the water phase; add 2 times to the water phase volume of n-hexane, fully oscillating and extracting, separating the n-hexane phase and the water phase; completely concentrating the n-hexane phase under reduced pressure to obtain a residue, and dissolving it in an appropriate amount of ethanol to obtain an ethanol liquid;

[0096] A4: Put the ethanol solution on a 300-400 mesh silica gel column (wherein, the mass ...

preparation example 1-1

[0097] Comparative Preparation Examples 1-1 to 1-3: Preparation of Honeysuckle Extract

preparation example 2

[0101] Preparation Example 2: Preparation of Dandelion Extract

[0102] B1: Add dandelion powder to 7.5 times the mass of ethanol, and then introduce CO 2 , sealed so that CO 2 The pressure is 5.3 MPa to form a swelling solvent system, and the temperature is raised to 55°C, and stirred and extracted at this temperature for 45 minutes; finally, the pressure is reduced to normal pressure, and naturally cooled to room temperature, filtered to obtain the filtrate;

[0103] B2: The filtrate is concentrated under reduced pressure completely to obtain the medicinal extract; the medicinal extract is dissolved with an appropriate amount of ethanol, and subjected to 300 mesh alumina column chromatography (wherein the mass ratio of the extract to the alumina column chromatography is 1:55) ), first rinse with acetone that is 3 times the volume of the column volume, then rinse with petroleum ether that is 1 times the volume of the column volume, and finally use a mixed solvent that is 2 t...

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Abstract

The invention relates to a traditional Chinese medicine compound extract for resisting RSV (respiratory syncytial virus) virus as well as a preparation method and an application thereof. The traditional Chinese medicine compound extract comprises a honeysuckle flower extract, a Mongolian dandelion herb extract, an indigowoad leaf extract, an indigowoad root extract, a red peony root extract, a root of largeleaf gentian extract, a fineleaf schizonepeta herb extract, a common lophatherum herb extract, licorice root, peppermint and weeping forsythia capsule. The traditional Chinese medicine compound extract has good RSV virus resistance, so that the traditional Chinese medicine compound extract can be used for treating and / or preventing various respiratory diseases caused by the RSV virus, and has broad research value and application prospect clinically.

Description

technical field [0001] The invention relates to an active ingredient of a traditional Chinese medicine and its preparation method and application, more specifically to an anti-RSV virus compound traditional Chinese medicine extract, its preparation method and application, and belongs to the technical field of extraction and application of traditional Chinese medicines. Background technique [0002] Research has found that respiratory syncytial virus (Respiratory syncytial virus, RSV virus) is the most important pathogen of lower respiratory tract infection in infants and young children, and is also the main factor of lower respiratory tract infection in the elderly and immunocompromised population. In this infection, RSV mainly damages the bronchiole epithelial cells, resulting in small airway obstruction, hyperinflation, and atelectasis, which can cause interstitial pneumonia and bronchiolitis. [0003] To control the virus, vaccines are usually the most effective means of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/899A61P31/14
Inventor 田景振崔清华侯林
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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