Virus-free tissue culture and rapid propagation method of Olea europaea
A technology of tissue culture rapid propagation and olive oil, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow seedling raising speed, and achieve the effects of improving disease resistance, improving quality, and rapid cultivation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0019] A method for rapid propagation of olives through detoxification tissue culture, comprising the following steps:
[0020] a. Seed shell removal: After mixing the olive seeds and gravel, put them in a blender, and remove the seed shells by ultra-high-speed stirring;
[0021] b. Making explants: soak the shelled seeds with 2-5% sucrose aqueous solution for 3-5 hours, take them out, dry them in the shade, and sterilize them, and use them as explants for detoxification tissue culture;
[0022] c. Induction culture: inoculate the seeds of step b on the induction medium to form a callus, and then transfer the callus to the differentiation medium to induce cluster buds to form rootless plantlets;
[0023] d. Proliferation culture: inoculate the rootless tissue seedlings induced above into the proliferation medium for subculture, subculture once in 30 days, and transfer continuously for 3 times, and the culture period is 100 days; the group of the proliferation medium The compo...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com