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Virus-free tissue culture and rapid propagation method of Olea europaea

A technology of tissue culture rapid propagation and olive oil, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow seedling raising speed, and achieve the effects of improving disease resistance, improving quality, and rapid cultivation

Inactive Publication Date: 2016-07-13
岳兰兰
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AI Technical Summary

Problems solved by technology

[0004] The existing olive cultivation is mainly hotbed cutting seedlings and hotbed seedlings, both of which can achieve the purpose of seedlings, but the slow speed of seedlings has become the main limiting factor

Method used

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Embodiment 1

[0019] A method for rapid propagation of olives through detoxification tissue culture, comprising the following steps:

[0020] a. Seed shell removal: After mixing the olive seeds and gravel, put them in a blender, and remove the seed shells by ultra-high-speed stirring;

[0021] b. Making explants: soak the shelled seeds with 2-5% sucrose aqueous solution for 3-5 hours, take them out, dry them in the shade, and sterilize them, and use them as explants for detoxification tissue culture;

[0022] c. Induction culture: inoculate the seeds of step b on the induction medium to form a callus, and then transfer the callus to the differentiation medium to induce cluster buds to form rootless plantlets;

[0023] d. Proliferation culture: inoculate the rootless tissue seedlings induced above into the proliferation medium for subculture, subculture once in 30 days, and transfer continuously for 3 times, and the culture period is 100 days; the group of the proliferation medium The compo...

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Abstract

The invention discloses a virus-free tissue culture and rapid propagation method of Olea europaea. The method comprises the following steps: 1, removing seed shells: removing seed shells through a sanding technology; 2, making explants: immersing the shelled seeds in a sucrose solution for 3-5h, taking out the immersed seeds, drying the immersed seeds in a dark place, and disinfecting the dried seeds; 3, carrying out inductive culture: inoculating the explants to an inductive culture medium to form callus tissues, transferring the callus tissues to a differentiation culture medium, and inducing clumpy buds to form rootless tissue seedlings; 4, carrying out propagation culture: inoculating the rootless tissue seedlings to a propagation medium, and carrying out subculture; 5, carrying out rooting culture: inoculating the rootless tissue culture seedlings to a rooting culture medium, and carrying out rooting culture; 6, carrying out seedling strengthening culture: carrying out seedling strengthening culture on the rooted tissue culture seedlings in a seedling strengthening culture medium for 10d; and 7, transplanting: tansplanting the seedlings after the virus-free tissue culture seedlings grow to 3-5cm, and culturing the transplanted seedlings to form young plants. The method is a new method for growing Olea europaea seedlings, accelerates the seedling growing speed, and meets scale production requirements.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture rapid propagation, in particular to a method for virus-free tissue culture rapid propagation of olives. Background technique [0002] Olive, also known as olean, is an evergreen tree belonging to Oleaceae and olean. It is native to the Mediterranean climate zone and was introduced to my country in the mid-19th century. It is the main oil tree species in Longnan, Gansu and Guangyuan, Sichuan. According to the use, it can be divided into five categories: oil, fruit, oil and fruit, pollination, and rootstock. Olive has no special requirements on the soil where it grows. As long as the pH value is between 6.5 and 8.0, the soil is loose and air-permeable, and it can grow no matter in siliceous soil or calcareous soil. But research shows that the best olive oil comes from the fruit of olive trees that are rich in calcium and have fertile soil. Strong winds and heavy rains during the flowe...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 岳兰兰
Owner 岳兰兰
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