Application of isorhamnetin to measurement of copper ions
A technology of isorhamnetin and copper ions, which is applied in the field of optical analysis and detection, can solve the problems of less research on fluorescence performance and unreported detection research, and achieve the effects of high sensitivity, low interference and low detection limit
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Embodiment 1
[0021] The application of isorhamnetin in measuring copper ions comprises the following steps:
[0022] (1) Accurately weigh 0.0316g of isorhamnetin, dilute it to a 10mL volumetric flask with methanol, and make a concentration of 1×10 -2 mol L -1 Solution, take 0.1mL and dilute to 100mL volumetric flask with PBS buffer solution (pH=7.40) to make 1×10 -5 mol L -1 Solution, ready for use; accurately weigh 0.0200g Cu(AC) 2 ·H 2 Dilute O to a 10mL volumetric flask with water to make a solution containing copper ions with a concentration of 1×10 -2 mol L -1 solution, and successively diluted to 1×10 -3 , 1×10 -4 , 1×10 -5 mol L -1 solution, ready to use.
[0023] (2) Prepare several 5mL dry and clean sample tubes, add 2mL1×10 -5 mol L -1 The isorhamnetin solution, and the copper ion solution with different volumes and different concentrations, the concentration of isorhamnetin is 1 × 10 -5 mol L -1 , the copper ion concentration is 0×10 -7 mol L -1 , 0.1×10 -7 mol ...
Embodiment 2
[0031] Containing isorhamnetin 1 x 10 -5 mol L -1 In the buffer, add Li-containing + 、Na + 、K + , Mg 2+ , Ca 2+ 、Cr 3+ , Mn 2+ , Fe 3+ 、Co 2+ 、Ni 2+ 、Cu 2+ , Zn 2+ , Pb 2+ 、Cd 2+ , La 3+ 、Ce 3+ 、Eu 3+ 、Gd 3+ 、Dy 3+ acetate solution, where Li + 、Na + 、K + , Mg 2+ , Ca 2+ Concentration is 5 times of copper ion concentration, Cr 3+ , Mn 2+ , Fe 3+ 、Co 2+ 、Ni 2+ , Zn 2+ , Pb 2+ 、Cd 2+ , La 3+ 、Ce 3+ 、Eu 3+ 、Gd 3+ 、Dy 3+ Concentration is 2 times of copper ion concentration, and the fluorescent intensity measured under the same condition and method of embodiment 1 is as follows image 3 As shown (other unmarked metal ions in the figure are from left to right: Li + 、Na + 、K + , Mg 2+ , Ca 2+ 、Cr 3+ , Mn 2+ , Fe 3+ 、Co 2+ 、Ni 2+ , Zn 2+ , Pb 2+ 、Cd 2+ , La 3+ 、Ce 3 + 、Eu 3+ 、Gd 3+ 、Dy 3+ ), it can be seen from the figure that only the addition of copper ions will cause obvious fluorescence quenching, and other metal ions have litt...
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