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A method of collecting chlorella cultured in pig farm waste water

A technology for pig farm wastewater and chlorella, which is applied in the field of microbial culture and collection, can solve the problems of restricting the industrialization process of microalgae oil production, unfavorable mass application, low culture density, etc., and achieves good economic benefits and reduces collection costs. , the effect of simple operation

Inactive Publication Date: 2016-07-06
CHINA AGRI UNIV
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Problems solved by technology

Second, because the microalgae cells are very small (2-20 microns), the culture density is low (0.5-5g / L), and the culture system contains a lot of water, so it is necessary to remove a considerable volume of algae cells from the culture medium. Water, the process is time-consuming and energy-consuming. According to estimates, the cost of microalgae harvesting may account for 20% to 30% of the entire microalgae biodiesel production cost, which restricts the industrialization process of microalgae oil production
But at present, the use of flocculation technology is mainly to collect microalgae in the culture medium (BG11). The cost of producing microalgae in BG11 medium is relatively high, which is not conducive to large-scale application in actual production. Therefore, there is an urgent need for a low-cost method of collecting microalgae. Methods

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  • A method of collecting chlorella cultured in pig farm waste water
  • A method of collecting chlorella cultured in pig farm waste water
  • A method of collecting chlorella cultured in pig farm waste water

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Embodiment 1

[0024] The cultivation of embodiment 1 chlorella 34-2 in pig farm waste water

[0025] 1. Chlorella sp. 34-2 is observed under a microscope. The algal strain is spherical or oval, with a diameter of 4-8um. Each cell has a goblet-shaped pigment body and a nucleus. The cell wall is thinner. The chlorella strain (Chlorellasp.) 34-2 was preserved on May 15, 2014 in the General Microbiology Center of the China Committee for the Collection of Microorganisms (CGMCC for short), address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing , Institute of Microbiology, Chinese Academy of Sciences, Zip Code 100101), the preservation number is CGMCCNo.9225, and the classification is named as Chlorella sp.

[0026] 2. Pig raising wastewater is taken from Hongyun Farm in Changping District. Before dilution, the wastewater is precipitated and filtered with gauze. See Table 1 for the total nitrogen, ammonia nitrogen, total phosphorus, COD and pH values ​​in pig wastewater.

[0...

Embodiment 2

[0031] The collection of embodiment 2 Chlorella 34-2

[0032] Configure flocculant mother liquor to make FeCl 3 .6H 2 O mother liquor concentration is 20g / L. The pH of the algae liquid obtained in Example 1 was adjusted with 1N NaOH or HCl solution, so that the pH of the algae liquid was 4, 5, 6, 7, 8, 9, 10, 11 and 12, respectively. Then, the FeCl 3 .6H 2 The O mother liquor was added to the above-mentioned algae liquors with different pH values ​​to form flocculants with final concentrations of 0, 200, 400, 500, 600, 800 and 1000mg / LFeCl respectively. 3 63 different treatments, the results show that at pH 6, FeCl in algae liquid 3 When the final concentration was 500mg / L, the flocculation efficiency of Chlorella algae was the highest, reaching 98% (see figure 1 , figure 2 ), at the same time, when the pH is 11, the FeCl in the algae liquid 3 When the final concentration is 500mg / L, the flocculation efficiency of Chlorella algae also reaches 98%, and the effect is eq...

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Abstract

The invention discloses a method of collecting chlorella cultured in pig farm waste water, and belongs to the field of microorganism culture and collection. The chlorella 34-2 the accession number of which is CGMCC NO.9225 can grow and produce biolipid by utilizing the pig farm waste water. After the chlorella is cultured in the pig farm waste water, through adjusting the pH value and adding iron trichloride, chlorella cells growing in the waste water can be flocculated, and the chlorella can be effectively collected. The method is simple in operation and low in cost, overcomes a restriction that a flocculation technique can only be used for collecting the chlorella in culture media, greatly reduces the collection cost, increases the collecting efficiency and has good economic benefit.

Description

technical field [0001] The invention belongs to the field of microorganism cultivation and collection, and in particular relates to a method for collecting chlorella cultured in pig farm wastewater capable of producing oil. Background technique [0002] Energy is an important material basis for the survival and development of human society, but with the rapid development of industry and economy, the problem of energy shortage is becoming more and more serious. Countries around the world are actively and extensively carrying out research on renewable energy including biomass energy. Microalgae is a good raw material for biodiesel production. It has the characteristics of strong environmental adaptability, high photosynthetic efficiency, short growth cycle, high biological yield, and high oil production rate. [0003] The key issue of oil production from microalgae is the cost issue. The high production cost makes the production of biodiesel from algae still at a disadvantage...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCY02E50/10
Inventor 陈三凤张波石皓文
Owner CHINA AGRI UNIV
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