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A magnetically stirred flow cytometer flow chamber

A flow cytometer and magnetic stirring technology, applied in the field of flow chamber, can solve the problems of sheath liquid in sheath liquid chamber, sample contamination of flow chamber, easy blocking of nozzles, etc., and achieves reduced instrument accuracy, large volume, and convenient casting. Effect

Active Publication Date: 2018-04-17
ANHUI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But they didn't solve the sheath fluid chamber. The sheath fluid starts to have velocity in the flow chamber, especially when the machine is turned on, it takes a long time to form a stable laminar flow.
Easy to clog the jet or contaminate the flow cell with sample during continuous injection

Method used

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  • A magnetically stirred flow cytometer flow chamber
  • A magnetically stirred flow cytometer flow chamber

Examples

Experimental program
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Embodiment 1

[0032] Such as figure 1 , figure 2 A flow cytometer flow chamber of a magnetic stirring type is shown, comprising a flow chamber, a sampling needle 1, and a sheath fluid chamber 3, wherein the sampling needle 1 passes through the flow chamber and the sheath fluid chamber 3, and the The sheath fluid chamber 3 is located above the flow chamber and connected through the sheath fluid hole 4 ; magnetic beads are arranged in the flow chamber, and an electromagnet 11 is arranged between the flow chamber and the sheath fluid chamber 4 .

[0033] Furthermore, the flow chamber is divided into flow chamber 5, flow chamber 2, and flow chamber 8 from top to bottom, and an upper width Lower narrow transition section 6 .

[0034] Further, the first flow chamber 5 , the second flow chamber 7 , and the third flow chamber 8 are all cylinders, and the cross-sectional area of ​​the first chamber 5 is smaller than that of the second chamber 7 ; the cross-sectional area of ​​the second chamber 7...

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Abstract

The present invention relates to a flow chamber of a flow cytometer, and in particular to a magnetic stirring flow chamber of a flow cytometer. It consists of a flow chamber, a sample needle, and a sheath liquid chamber. The sample needle passes through the flow chamber and the sheath liquid chamber. The sheath liquid chamber is located above the flow chamber and connected through the sheath liquid hole; the flow chamber Magnetic beads are provided, and an electromagnet is provided between the flow chamber and the sheath liquid chamber. The upper end of the sheath liquid chamber has a sheath liquid inlet. It can quickly stabilize laminar flow and save energy. When the nozzle is ejected, the sheath liquid still has centripetal force. The injection needle is located at the center of the circle and the eye of the typhoon, which ensures that it will fall straight down and will not mix with the sheath liquid or block the nozzle.

Description

technical field [0001] The utility model relates to a flow chamber of a flow cytometer, in particular to a flow chamber of a magnetic stirring type flow cytometer. Background technique [0002] Flow cytometer is a very common detection instrument. The flow chamber in its liquid circuit system is composed of sample tube, sheath liquid tube and nozzle. It is usually made of transparent and stable materials such as optical glass and quartz. The design and production are very fine, it is the heart of the fluid flow system. The sample tube stores the sample, and a single cell suspension is ejected from the sample tube under the action of the liquid flow pressure; the sheath liquid flows from the sheath liquid tube from the surrounding to the nozzle hole, surrounds the sample and then ejects from the nozzle to ensure a stable liquid flow. Due to the effect of the sheath fluid, the detected cells are confined to the axis of the fluid flow. Typically, the sample is injected into t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14
CPCG01N15/1404G01N15/1409
Inventor 闫浩马晓艳林凌张庭廷
Owner ANHUI NORMAL UNIV
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