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Dendrobium officinale tissue culture method based on enclosed-type plant factory

A technology of Dendrobium officinale and Dendrobium group is applied in the field of cultivating seedlings of Dendrobium officinale group, and can solve the problems of inability to precisely control environmental factors for cultivating seedlings of Dendrobium officinale group and the like.

Inactive Publication Date: 2016-06-29
TIANJIN ZHENFA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing group cultivation of Dendrobium candidum is mainly in the greenhouse, and the environmental factors of the group cultivation of Dendrobium candidum cannot be accurately controlled, but in the closed plant factory, the environmental factors of the group cultivation of Dendrobium candidum can be precisely controlled

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] Environmental conditions: In a closed plant factory, the temperature is 22±1°C, the humidity is 65±5%, and the CO2 concentration is 800±50 micromol / mol;

[0018] Training conditions:

[0019] Culture container: 380ml square polycarbonate culture container with two 10mm ventilation holes on the cover;

[0020] Medium: MS+0.5mg / L6BA+0.05mg / LNAA, add 2% sucrose and 08% agar to the basic components of MS medium;

[0021] Medium pH value: 5.8~5.9;

[0022] Culture time: 12 weeks;

[0023] Lighting equipment: 60w LED tricolor lamp;

[0024] Lighting time: 12 hours / day

[0025] Test parameters:

[0026] Morphological indicators: plant height, stem diameter, dry weight, fresh weight, number of axillary buds;

[0027] Physiological indicators: net photosynthetic rate, stomatal conductance, transpiration rate, chlorophyll content;

[0028] Set three regions according to the light intensity: 37 micromol / m² sec, 68 micromol / m² sec and 92 micromol / m² sec;

[0029] After 12 w...

example 2

[0031] Environmental conditions: In a closed plant factory, the temperature is 22±1°C, the humidity is 65±5%, and the CO2 concentration is 800±50 micromol / mol;

[0032] Training conditions:

[0033] Culture container: 380ml square polycarbonate culture container with two 10mm ventilation holes on the cover;

[0034] Medium: MS+0.5mg / L6BA+0.05mg / LNAA, add 2% sucrose and 08% agar to the basic components of MS medium;

[0035] Medium pH value: 5.8~5.9;

[0036] Culture time: 12 weeks;

[0037] Lighting equipment: 60w LED tricolor lamp;

[0038] Illumination intensity: 68 micromole / square meter second

[0039] Test parameters:

[0040] Morphological indicators: plant height, stem diameter, dry weight, fresh weight, number of axillary buds;

[0041] Physiological indicators: net photosynthetic rate, stomatal conductance, transpiration rate, chlorophyll content;

[0042] Set three areas according to the light time: 9 hours / day, 12 hours / day, 15 hours / day;

[0043] After 12 w...

example 3

[0045] Environmental conditions: In a closed plant factory, the temperature is 22±1°C, the humidity is 65±5%, and the CO2 concentration is 800±50 micromol / mol;

[0046] Training conditions:

[0047] Culture container: 380ml square polycarbonate culture container with two 10mm ventilation holes on the cover;

[0048] Medium: MS+0.5mg / L6BA+0.05mg / LNAA, add 2% sucrose and 08% agar to the basic components of MS medium;

[0049] Medium pH value: 5.8~5.9;

[0050] Culture time: 12 weeks;

[0051] Lighting time: 12 hours / day

[0052] Illumination intensity: 68 micromole / square meter second

[0053] Test parameters:

[0054] Morphological indicators: plant height, stem diameter, dry weight, fresh weight, number of axillary buds;

[0055] Physiological indicators: net photosynthetic rate, stomatal conductance, transpiration rate, chlorophyll content;

[0056] Set up three areas according to the lighting equipment: LED single-color light area, fluorescent light area, LED three-co...

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Abstract

The invention discloses a dendrobium officinale tissue culture method based on an enclosed-type plant factory. The method comprises the following steps: setting the environment condition of a tissue-culture room in the enclosed-type plant factory, selecting the tissue culture plants, selecting a medium, setting the illumination intensity and the illumination time of a LED artificial light-supplement lamp of the enclosed-type plant factory, and proportioning the lamp beads of red, blue and far-red light; so that the propagation speed of the dendrobium officinale tissue culture plants is high, tissue culture plants hardening seedling and transplanting survival rate can be increased, and culture period of the tissue culture plants can be shortened.

Description

technical field [0001] The invention belongs to the field of agricultural science and technology planting, and relates to a method for cultivating seedlings of Dendrobium candidum group based on a closed plant factory. Background technique [0002] Dendrobium officinale is a rare and endangered medicinal plant with a special growth environment and slow growth in the natural environment. In recent years, wild resources are on the verge of extinction and the demand is increasing year by year. The current artificial cultivation yield and quality are far from meeting market demand. Therefore, improving the existing artificial cultivation mode, promoting the large-scale and industrialized development of Dendrobium officinale, and rapidly improving its cultivation yield and quality will help to achieve the unification of economic and social benefits. The use of closed plant factories to study the physiological characteristics and growth environment of Dendrobium candidum is the b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 黄刚
Owner TIANJIN ZHENFA TECH
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