Preparation method of donkey-hide gelatin small molecular peptide
A technology of small molecule peptides and donkey-hide gelatin, which is applied in the field of preparation of donkey-hide gelatin small-molecule peptides, can solve problems such as indigestion and absorption, increasing the burden of gastrointestinal digestion, and difficulty in achieving drug efficacy due to poor digestion and absorption of donkey-hide gelatin.
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Embodiment 1
[0024] A preparation method of donkey-hide gelatin small molecule peptide, comprising the following steps:
[0025] (1) Raw material pretreatment: Carry out vacuum drying to the donkey-hide gelatin raw material until the water content is 4.8%, then pulverized into donkey-hide gelatin fine powder with a particle diameter of 150 microns;
[0026] (2) Enzymolysis: add water 13 times the weight of donkey-hide gelatin fine powder to donkey-hide gelatin fine powder, heat to 95°C after mixing evenly, keep warm for 15 minutes, then cool down to 50°C, adjust pH to 8.5, add alkaline protease for the second step For one enzymolysis, the amount of alkaline protease used is 1% of the weight of donkey-hide gelatin fine powder; then keep the temperature at 50°C, adjust the pH to 7.3, add neutral protease to carry out the second enzymolysis, the use amount of neutral protease is 1% of the weight of donkey-hide gelatin fine powder; finally keep the temperature at 50°C, adjust the pH to 3.5, ad...
Embodiment 2
[0031] A preparation method of donkey-hide gelatin small molecule peptide, comprising the following steps:
[0032] (1) Raw material pretreatment: The donkey-hide gelatin raw material was vacuum-dried at 55° C. for 16 hours until the moisture content was 5%, and then crushed into donkey-hide gelatin fine powder with a particle size of 150 microns.
[0033] (2) Enzymolysis: add water 12 times the weight of donkey-hide gelatin fine powder to donkey-hide gelatin fine powder, heat to 96°C after mixing evenly, keep warm for 16 minutes, then cool down to 48°C, use 10% NaOH solution to adjust the pH to 8.6, add Bacillus subtilis alkaline protease for the first enzymolysis, the amount of alkaline protease used is 1% of the weight of donkey-hide gelatin fine powder, the activity of alkaline protease is 500000u / g, and the enzymatic hydrolysis temperature of alkaline protease is 48°C , the enzymolysis time of alkaline protease is 3 hours; then the temperature is raised to 50°C, the pH is...
Embodiment 3
[0038] A preparation method of donkey-hide gelatin small molecule peptide, comprising the following steps:
[0039] (1) Raw material pretreatment: The donkey-hide gelatin raw material was vacuum-dried at 50° C. for 17 hours until the water content was 5%, and then crushed into donkey-hide gelatin fine powder with a particle size of 200 microns.
[0040] (2) Enzymolysis: add water 10 times the weight of donkey-hide gelatin fine powder to donkey-hide gelatin fine powder, heat up to 98°C after mixing evenly, keep warm for 10 minutes, then cool down to 55°C, use 10% NaOH solution to adjust the pH to 9.0, adding Bacillus subtilis alkaline protease to carry out the first enzymolysis, the usage amount of alkaline protease is 1% of donkey-hide gelatin fine powder weight, the activity of alkaline protease is 500000u / g, the enzymolysis temperature of alkaline protease is 55 ℃ , the enzymolysis time of alkaline protease is 2.5 hours; then keep the temperature at 55°C, use 10% HCl solutio...
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