Method for preparation of lentinan and lentinus edodes polypeptide from defective lentinus edodes and lentinus edodes stems

Abstract

The invention belongs to a method for preparation of lentinan and lentinus edodes polypeptide from defective lentinus edodes and lentinus edodes stems. The method includes: crushing defective lentinusedodes and lentinus edodes stems, carrying out ultrasonic low-temperature continuous countercurrent extraction on the crushed lentinus edodes, carrying out biological enzymolysis on the extracted solution, carrying out enzyme deactivation treatment on the enzymatic hydrolysate, conducting filtering, fine filtering and re-separating on the enzymatic hydrolysate subjected to enzyme deactivation treatment to separate a polysaccharide concentrated solution and a polypeptide concentrated solution, and carrying out spray drying on the polysaccharide concentrated solution and the polypeptide concentrated solution respectively to prepare lentinan and lentinus edodes polypeptide. The method has the characteristics of easily available raw materials, low cost, few regional limitation of raw materials, and lower cost of the prepared lentinan relative to water extraction and alcohol precipitation method, can realize co-production of the lentinan and lentinus edodes polypeptide through a same set of process, and the lentinan and lentinus edodes polypeptide obtained through co-production have the advantages of high purity, few impurity, stable quality, no solvent residue, easy absorption and easy storage.

Description

technical field [0001] The invention belongs to the technical field of shiitake mushroom deep processing, and in particular relates to a method for preparing lentinan and shiitake polypeptide from defective shiitake mushrooms and shiitake stalks. Background technique [0002] Lentinus edodes is the second largest edible fungus in the world after the twin mushrooms, and is known as the "King of Mushrooms". Shiitake mushrooms are known as "mountain delicacy" among the people. It can be used as both medicine and food. Its medicinal function is that the anti-cancer effect of shiitake mushrooms is the best. It is rich in lentinan, and its nutritional value is full of 18 kinds of amino acids needed by the human body, vitamin D to promote the absorption of human calcium and triterpenoids. In particular, the rich leucine and lysine are lacking in most grain proteins and vegetable proteins. [0003] my country is the main producer of shiitake mushrooms in the world. The shiitake mu...

AI Technical Summary

Problems solved by technology

[0003] my country is the main producer of shiitake mushrooms in the world. The shiitake mushrooms produced are mainly sold in dry and fresh products in Europe, Japan and Southeast Asian countries. The deep processing is relatively small. At present, the rough processing mainly uses shiitake mushroom crude polysaccharide as the main component to enter the market, and the added value of the product is low. Moreover, most of the effective substances in the crude polysaccharides of shiitake mushrooms cannot be directly absorbed by the human body.

The deep-processing products in shiitake mushrooms include lentinan and lentinan polypeptide. Lentinan is mainly dextran, containing a small amount of glucose and xylose, with an average molecular weight of about 500,000 Daltons; lentinan polypeptide is the main biologically active substance in shiitake mushrooms. It is an ideal immune enhancer, which has the functions of improving human immunity, inhibiting tumors, reducing transaminases, protecting the liver and lowering cholesterol; however, the recovery rate of lentinan and lentinan polypeptide is currently low, resulting in high costs, and the recovery of both It cannot be realized through the same process, resulting in high purchase and control costs for enterprises, and it is impossible to achieve large-scale mass production