Method for extracting and separating labdane diterpenes from Himalayan mayapple fruit and application thereof
A technology for compounds and diterpenoids, applied in the separation/purification of hydroxy compounds, separation/purification of carbonyl compounds, organic chemical methods, etc., can solve the problems such as the purity of the diterpenoids that have not been seen yet, Achieve significant economic and social benefits, easy to operate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0015] In the specific implementation of the present invention, 9 kg of lotus lobata is heated and refluxed for 3 times with ethanol with 3 times the weight of lotus lobes and a volume concentration of 95%, and the extraction temperature is 95° C., and the extraction time is 1.5 hours. Paste ethanol extract, suspend the ethanol extract in 3.2L distilled water, extract 3 times with petroleum ether and dichloromethane successively, 3.2L each time; put the dichloromethane extraction part on a silica gel column, and use volume ratio Carry out gradient elution for 100:0, 100:5, 100:7, 100:10, 100:30 petroleum ether-acetone mixed solvents, each gradient uses 15L eluent, and the flow rate is 15mLmin -1 , each 300ml volume is a fraction, collect 215 fractions, each fraction uses a GF254 thin-layer plate, uses petroleum ether-acetone with a volume ratio of 1:1 as a developer, and a sulfuric acid-ethanol solution with a volume ratio of 10:90 as Chromogenic agent, heated at 105°C for 5mi...
Embodiment 2
[0017] In the specific implementation of the present invention, 6 kg of lotus lobes can also be heated and refluxed for 3 times with ethanol with 5 times the weight of lotus lobes and a volume concentration of 75%, the extraction temperature is 92°C, the extraction time is 1.8 hours, and the ethanol is recovered under reduced pressure to obtain For extract-like ethanol extract, suspend the ethanol extract in 2L of distilled water, extract with petroleum ether and dichloromethane three times, 2L each time; put the dichloromethane extraction part on a silica gel column, and use 100:0, 100:5, 100:7, 100:10, 100:30 petroleum ether-acetone mixed solvents for gradient elution, each gradient uses 10L eluent, the flow rate is 10mLmin -1 , every 200ml volume is a fraction, collect 215 fractions, each fraction uses a GF254 thin-layer plate, uses petroleum ether-acetone with a volume ratio of 1:1 as a developer, and a sulfuric acid-ethanol solution with a volume ratio of 10:90 as a Chrom...
Embodiment 3
[0019] In the specific implementation of the present invention, 7 kg of lotus lobes can also be heated and refluxed for 3 times with ethanol with 4 times the weight of lotus lobes and 80% volume concentration, the extraction temperature is 93° C., the extraction time is 2 hours, and the ethanol is recovered under reduced pressure to obtain For extract-like ethanol extract, suspend the ethanol extract in 2.5L of distilled water, extract with petroleum ether and dichloromethane three times, 2.5L each time; put the dichloromethane extraction part on a silica gel column, and use volume Gradient elution was carried out with petroleum ether-acetone mixed solvents with ratios of 100:0, 100:5, 100:7, 100:10, and 100:30. Each gradient used 13L eluent with a flow rate of 13mLmin. -1 , every 260ml volume is a fraction, collect 215 fractions, each fraction uses a GF254 thin-layer plate, uses petroleum ether-acetone with a volume ratio of 1:1 as a developer, and a sulfuric acid-ethanol solu...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com