Human lp-pla2 biotin-streptavidin fluorescence immunochromatography detection card and preparation method thereof

Abstract

The invention discloses a human Lp-PLA2 biotin-streptavidin fluorescence immunochromatographic assay card and a preparation method thereof and aims at providing an assay card which is supportive of quick combining, free of outside interference during reaction and high in specificity and stability and a preparation method thereof. The technical scheme includes that assay test paper comprises a substrate, a sample pad, a mark pad, a coated film and an absorption pad are connected on the substrate. The assay card is characterized in that an anti-Lp-PLA2 monoclonal antibody 1 of fluorescent microspheres and a biotin anti-Lp-PLA2 monoclonal antibody 2 are coupled on the mark, a streptavidin assay line T and a goat-anti-mouse polyclonal antibody quality control line C are parallelly arranged on the coated film. The invention belongs to the field of fluorescence immunochromatographic assay.

Description

technical field [0001] The invention discloses a detection reagent card, specifically, a detection reagent card for detecting lipoprotein-related phospholipase A2 in samples such as human serum, plasma or whole blood, belonging to the field of fluorescence immunochromatography detection. Background technique [0002] In recent years, with the improvement of people's living standards, the incidence and mortality of cardiovascular and cerebrovascular diseases have been increasing year by year. The cardiovascular and cerebrovascular report pointed out that the number of patients with cardiovascular and cerebrovascular diseases in my country has reached 290 million, and one person in every five adults suffers from cardiovascular and cerebrovascular diseases, and one person dies of cardiovascular and cerebrovascular diseases every 10 seconds. It is urgent to treat cardiovascular and cerebrovascular diseases. [0003] The basis of cardiovascular and cerebrovascular diseases is ath...

AI Technical Summary

Problems solved by technology

[0007] (1) Enzyme-linked immunoassay method, the detection sensitivity of this method is high, but the operation process is cumbersome and the measurement time is long, so it is not suitable for clinical application

[0008] (2) continuous monitoring method, this method is to measure enzyme activity according to the characteristic of enzyme and quantitative principle, yet many factors can interfere with enzymatic reaction process, as time, temperature, pH, anticoagulant etc.; Strict concentration requirements and expensive equipment

This method has high sensitivity, but is cumbersome to operate, requires a special luminescent substrate-antigen / antibody conjugate, is prone to cross-contamination, requires large analytical instruments, and has high reagent costs, which limit its large-scale development and use.

[0010] (4) Colloidal gold detection method, the method is simple to operate, but the sensitivity is low, especially when the concentration of the measured substance in the sample is too low, it is likely to miss the detection, which is not conducive to the prevention of cardiovascular embolism diseases

The fluorescence lifetime of these fluorescent substances is relatively short, the Stokes shift is small, only tens of nanometers, and the excitation spectrum and emission spectrum usually partially overlap, causing serious mutual interference.

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