White paeony root medicinal material extraction and purification process
A technology of peony and medicinal materials, applied in the field of medicinal material extraction, to achieve the effect of easy operation
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Embodiment 1
[0012] Embodiment 1, the extraction and purification process research of Radix Paeoniae Alba medicinal material, comprise following steps:
[0013] A, ethanol extraction Take 50g of herbal decoction pieces of Radix Paeoniae Alba, add 400g of 70% ethanol solution, extract for 90min, repeat 3 times, filter, and combine the extracts;
[0014] B, thin-layer chromatography carry out thin-layer chromatography with the extract of Radix Paeoniae Alba that extraction obtained in previous step, condition is silica gel 6254 plate; Developing agent: chloroform-methanol (5:1); Reference substance: Paeoniae officinale;
[0015] C: the extract of Radix Paeoniae Alba that the first step obtains is concentrated to 0.5g / ml, filter paper suction filtration, continue to concentrate to 2g crude drug / ml;
[0016] D: D101 type macroporous adsorption resin extraction and purification separation
[0017] First, take macroporous adsorption resins D101, H103, and HPD500 and soak them in 95% ethanol for...
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