Living single cell Raman analysis platform digital control system and method

Abstract

The invention relates to the field of single cell sorting, particularly, a digital control system and a digital control method for a living single cell Raman analytic platform. The system comprises an instrument control module, a cell image recognizing and analyzing module, a Raman analysis treatment module and a single cell screening control module. The digital control method includes the following steps: a) inputting a sample, collecting data, and performing edge detection and coordinate recognition; b) controlling an X axis, a Y axis and a Z axis to move to the position that the cell is located by means of the instrument control module according to the coordinate of the cells; c) comparing a single cell Raman spectrum with a Raman spectrum database to obtain information of the single cells; d) when the single cells satisfying conditions arrives at a selection branch position of a microfluidic chip, controlling an electromagnetic valve to push the single cells into a cell screening outlet by means of the instrument control module, or otherwise, enabling the single cells to flow into a waste liquid tank. The system and the method can form a systematized and complete operation platform for comprehensively supporting a control part of a high-throughput in-situ living single cell optical tweezers screening system, thereby promoting researching on single cell analysis.

Description

technical field [0001] The invention relates to the field of single cell sorting, in particular to a digital control system and method for a live single cell Raman analysis platform. Background technique [0002] Single-cell analysis is of great significance to the early diagnosis, treatment, drug screening and research of cell physiology and pathological process of major diseases, and has become one of the research hotspots. Due to the extremely small size of the cells, the diameter is generally 5-500 μm, the volume is fL-nL, the component content is small (fmol-zmol), and there are many types, which makes manipulation and analysis more difficult. Therefore, to carry out research at the single-cell level, cell sorting becomes a necessary prerequisite. [0003] The identification and sorting of single cells is based on the correct collection of indicators that characterize cell information. In the existing traditional flow cytometer, the identification of cell and particle...

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Problems solved by technology

[0005] Aiming at the problems of time and precision deviation and low efficiency in sorting single cells at present, and at the same time solving the problems of low information acquisition rate of the target by existing cell sorting equipment, the technical problem to be solved by the present invention is to provide a method that can The digital control system and method of the living single cell Raman analysis platform for automatic and intelligent image Raman acquisition, analysis and sorting of living single cells, especially suitable for in situ cell sorting of living single cells

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