Test method for studying high-temperature stress on wheat

Abstract

The invention relates to a test method for studying high-temperature stress on wheat, and belongs to the field of plant seedling high-temperature stress tests. The test method comprises the following steps: seedling culture, variety selection, sodium hydrosulfide solution preparation, sodium hydrosulfide solution spraying, sodium hydrosulfide solution concentration optimization, sodium hydrosulfide solution spraying time and frequency optimization and the like, wherein seedling culture comprises seed selection, soaking of seeds in clean water, seed soaking and sterilization, seed soaking at a constant temperature, germination acceleration, secondary seed selection, seed arrangement, seedling acceleration, seed picking and seedling. Seedling selection and optimization is performed multiple times in the period from seed soaking to seedling transplanting during seedling, therefore, consistency of initial test materials is guaranteed. The sodium hydrosulfide solutions of different concentration are sprayed to wheat seedlings cultivated for 20 days for high-temperature stress relieving tests, and concentration, spraying time, spraying frequencies of the sodium hydrosulfide solutions are optimized; besides, in combination with optimization and innovation of a sampling method during material drawing in the test, the accuracy of final test data can be better guaranteed from test design, procedures and results, therefore, great benefit is provided for final disclosure of physiological and biochemical changes after test treatment.

Description

technical field [0001] The invention relates to the technical field of high temperature stress test of plant seedlings, in particular to a test method for studying high temperature stress of wheat. Background technique [0002] In the current high temperature stress test of wheat seedlings, the success or failure of the seedling growth consistency test is limited by the existing seedling cultivation methods. When cultivating seedlings on solid medium, it can only be checked during the seed selection process, that is, the selection of size, Seeds with the same color and plumpness are expected to breed wheat seedling materials with small differences, so as to ensure the uniformity and accuracy of the test materials as much as possible. However, when solid medium is used to cultivate seedlings, the phenomenon of lack of seedlings and excessive number of deformed seedlings often occurs, and the uniformity of the test materials used for the test treatment cannot be guaranteed, wh...

AI Technical Summary

Problems solved by technology

However, when using solid medium to cultivate seedlings, there will often be a lack of seedlings and more deformed seedlings, and the uniformity of the test materials used for the test treatment cannot be guaranteed, which will bring difficulties and inconvenience to the follow-up high temperature stress test operation.

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