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Cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese

A technology of cutting propagation and rooting rate, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve the problems of long dormant period after seed embryo maturation, irregular seed germination, and small seed setting and other problems, to achieve the effect of short seedling time, high rooting rate and favorable growth

Inactive Publication Date: 2016-05-04
BEIJING UNIV OF AGRI
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  • Application Information

AI Technical Summary

Problems solved by technology

Liriodendron is a slow-growing tree species, and it is a dioecious plant. The seed setting is small, and the post-ripening dormant period of the embryo is long, and there are problems such as irregular seed germination and low germination rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A cutting propagation method for improving the rooting rate of Liriodendron tulipifera cuttings. Collect 2-year-old thick branches for fringe production. The length of the cuttings is 5 cm, and 3 leaves are left; the cuttings are placed in a greenhouse with a temperature of 20°C and a relative humidity of 90%. Let it stand for 12 hours, then insert the cuttings into the triangular flask containing the medicinal solution for dark cultivation, the insertion depth is 3cm, and apply ultrasonic waves with a frequency of 25kHz to the triangular flask at the same time, the ultrasonic waves work for 5 seconds, stop for 5 seconds, and work for 8 minutes in total; The medicinal solution is prepared by mixing 0.2wt% benzyl adenine, 0.1wt% plant vitamin C, 0.3wt% boric acid, 0.15wt% citric acid, and water;

[0017] After standing still, use NAA+2, 4-D mixed solution to soak the 4 cm of the cuttings base, and use an ultrasonic cell breaker to ultrasonically treat the cuttings while s...

Embodiment 2

[0023] This embodiment adopts the method of embodiment 1, and the difference from embodiment 1 is: the power of the ultrasonic cell disruptor is 100-150w; every time the ultrasonic wave works for 5s, it stops for 6s, and works for a total of 8min; The time is 4 minutes. The concentration of NAA was 2000 ppm and the concentration of 2,4-D was 600 ppm.

[0024] The comparison group 1-2 adopts the rooting method of the embodiment 1-2 corresponding to its number respectively, and the difference is that the samples in the comparison group do not use ultrasonic treatment. The clear water control group adopts the rooting method of embodiment 1, and the difference is: the rooting powder treatment solution is not used to soak, but the clear water is used to soak for 6 hours, and the rooting rate after ultrasonic treatment and without ultrasonic treatment is 0.

[0025] Table 1 Effects of different hormones and ultrasonic treatment on the rooting rate of American hickory cuttings

[0...

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Abstract

Provided is a cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese. The method is characterized by comprising the following steps: acquiring 2-5 years old thick branches and making the branches into cuttings, after standing completes, using NAA+2,4-D mixed liquor to soak 0-4 cm of cutting bases, while soaking, using an ultrasonic cell crusher to perform ultrasonic treatment on the cuttings, establishing a full-automatic mist-spraying cutting pool, paving a medium which is disinfected in the cutting pool, putting the cuttings in the medium, after cuttage, using a watering can to watering, to make the cuttings tightly contact with the medium, and performing management after cuttage.

Description

technical field [0001] The invention relates to a cultivation method, in particular to a cutting propagation method for improving the rooting rate of Liriodendron tulipifera cuttings. Background technique [0002] The preservation of Liriodendron resources and the rapid breeding of seedlings are the basis for solving the construction of paclitaxel forests, and the construction of large-scale Liriodendron plantations is the key to solve the problem of paclitaxel raw materials. Liriodendron is a slow-growing tree species, and it is a dioecious plant. The amount of seed setting is small, and the post-ripening dormant period of the embryo is long, and there are problems such as irregular seed germination and low germination rate. However, the recent development of raw material forests of Liriodendron tulipifera is based on the strategy of intensive planting at the seedling stage, so the propagation of large-scale cuttings is the basis for realizing this strategy. [0003] The o...

Claims

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Application Information

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IPC IPC(8): A01G9/10A01N59/14A01N43/90A01N43/08A01N37/36A01N39/04A01N37/10A01P21/00
CPCA01G9/0299A01N37/10A01N37/36A01N39/04A01N43/08A01N43/90A01N59/14A01N2300/00
Inventor 崔金腾张克中丁榕曹潇
Owner BEIJING UNIV OF AGRI
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