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Method for cloning AhbHLH1L genes and expressing functions for peanuts under adversity stress

A technology of adversity stress and expression method, applied in the field of adversity stress AhbHLH1L gene cloning and functional expression in peanut

Active Publication Date: 2016-04-13
SHANDONG PEANUT RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the cloning and functional research of bHLH transcription factors in peanuts. In this study, we found that the expression of this gene was significantly up-regulated in peanut salt-treated chips through microarray hybridization, and then carried out gene cloning and functional research on this gene.

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  • Method for cloning AhbHLH1L genes and expressing functions for peanuts under adversity stress
  • Method for cloning AhbHLH1L genes and expressing functions for peanuts under adversity stress
  • Method for cloning AhbHLH1L genes and expressing functions for peanuts under adversity stress

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Embodiment Construction

[0024] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, but it is not intended to further limit the present invention.

[0025] The concrete implementation process of the present invention is:

[0026] 1 Experimental materials and methods

[0027] 1.1 Experimental materials

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Abstract

The invention discloses a method for cloning AhbHLH1L genes and expressing functions for peanuts under adversity stress. The method is characterized by mainly comprising steps of (1), preparing and processing materials; (2), extracting RNA (ribonucleic acid) and synthesizing cDNA [complementary DNA (deoxyribonucleic acid)]; (3), cloning the genes. The method has the advantages that expression modes of the AhbHLH1L genes at low temperatures and under salt stress and drought stress are verified by means of fluorescence quantitative PCR (polymerase chain reaction), and the fact that the stress resistance of plants can be obviously improved by the AhbHLH1L genes is declared.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a method for cloning and functional expression of AhbHLH1L gene under adversity stress in peanuts. Background technique [0002] Peanut is rich in oil and protein, and is an important oil crop and economic crop in my country. The yield and quality of peanuts are seriously affected by drought, saline-alkali and other stresses, and the annual reduction rate of peanuts caused by drought in the whole country reaches more than 20%. However, due to the narrow genetic basis of peanut variety resources and the lack of high drought-resistant, cold-resistant, and salt-tolerant gene sources, it is difficult to breed high-resistant varieties using conventional breeding methods. With the rapid development of molecular biology, the use of transgenic technology to improve plant stress tolerance has achieved remarkable results in recent years, and a deeper understanding of stress-related genes and sig...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12N15/29C07K14/415
Inventor 陈娜禹山林迟晓元
Owner SHANDONG PEANUT RES INST
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