Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for the industrial production of flour from lipid-rich microalga biomass with no "off-notes" by controlling the oxygen availability

A biomass and utilization technology, applied in biochemical equipment and methods, specific-purpose bioreactor/fermenter, biomass post-treatment, etc.

Inactive Publication Date: 2016-04-06
CORBION BIOTECH INC
View PDF7 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0040] Therefore, there remains an unmet need for a process with guaranteed industrial-scale production of compositions of microalgae flour of the genus Chlorella genus having suitable organoleptic qualities, enabling their use in a greater number of more diverse food products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for the industrial production of flour from lipid-rich microalga biomass with no "off-notes" by controlling the oxygen availability
  • Method for the industrial production of flour from lipid-rich microalga biomass with no "off-notes" by controlling the oxygen availability
  • Method for the industrial production of flour from lipid-rich microalga biomass with no "off-notes" by controlling the oxygen availability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0138] Example 1: Production of lipid-rich Chlorella protothecoides - controlled oxygenation

[0139] The algal species used is Chlorella protothecoides UTEX250

[0140] pre-culture :

[0141] - 500 ml medium in a 2 l Erlenmeyer flask (Erlenmeyer flask);

[0142] -Composition of the culture medium:

[0143] Table 1.

[0144]

[0145]

[0146] Incubation was performed under the following conditions: time: 72 h; temperature: 28° C.; shaking: 110 rpm (Infors Multitron incubator).

[0147] The preculture was then transferred to a Sartorius 301 fermenter.

[0148] Cultivation for biomass production:

[0149] The base medium is as follows:

[0150] Table 2.

[0151]

[0152]

[0153] The initial volume (Vi) of the fermentor was adjusted to 7 l after inoculation. A final volume of 15-20l is reached.

[0154] The parameters used to run the different tests are as follows:

[0155] table 3.

[0156]

[0157] When the residual concentration of glucose fell ...

Embodiment 2

[0179] Example 2: Sensory Evaluation of 3 Batches Produced in Example 1

[0180] In this example, the applicant company proposes to determine the organoleptic quality of microalgal flour prepared from 3 batches produced in Example 1, which have been ground and dried according to the method described in patent application WO2010 / 12093 from Said flour made from biomass.

[0181] In French Patent Application No. 1356113, the applicant company defined a very simple tasting matrix that allows sensory evaluations similar to those obtained with far more complex and very different formulations (e.g. ice cream or brioche) sensory evaluation. The precision or accuracy of the assessment using this tasting matrix is ​​much higher than that performed using a simple aqueous solution, which has proven unable to predict the organoleptic qualities of, for example, microalgal flour compositions in ice cream.

[0182] Thus, the microalgal flour tasting composition includes:

[0183] - 7% co...

Embodiment 3

[0232] Embodiment 3: Different from the 3 batches of the microalgal flour composition obtained from Example 1 Analysis of Volatile Organic Compounds (VOCs) Related to Sensory Classification of Acceptable Abnormal Characteristics

[0233] To carry out the SPME / GC-MS analysis of 3 batches of microalgal flour compositions obtained from the biomass produced in Example 1, the method was carried out as described below.

[0234] A test sample of 3 g aliquots was introduced into a sealed SPME flask (20 ml) and incubated at 60°C for 15 min, and then at 60°C using DVB / CAR / PDMS (divinylbenzene / carboxan / polydimethylsiloxane Abbreviation for oxane, df50 / 30μm) SPME fiber extraction for 45min.

[0235] The extracted VOCs were desorbed in an injector of a TSQGC-MS system from Thermo Scientific and then separated on a CPwax52 (60m x 0.25mm, 0.25μm) column using helium at 1.5ml / min.

[0236] The temperature program is as follows: keep at 50°C for 3 minutes, then program the temperature up ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for fermentative production, on an industrial scale, of lipid-rich biomass of microalgae of the Chlorella genus having acceptable sensory properties, characterized in that the dissolved oxygen availability in the fermenter is controlled by tracking the respiratory quotient of said microalgae.

Description

[0001] The present invention relates to a novel process for the industrial scale production of biomass of lipid-rich microalgae of the genus Chlorella with acceptable organoleptic quality. [0002] The present invention is particularly suitable for controlling the oxidative rancidity associated with the instability over time of microalgal biomass rich in monounsaturated fatty acids, specifically oleic acid. Background technique [0003] Historically, algae "just need water and sunlight" to grow, so algae have long been considered a source of food. [0004] There are several species of algae that can be used in food, mostly "macroalgae" such as kelp, sea cabbage (Ulvalactuca) and Porphyra (cultivated in Japan) or "dulse" (Palmaria palmata) type red algae. [0005] In addition to these macroalgae, however, there are other sources of algae represented by "microalgae", that is, photosynthetic or non-photosynthetic single-celled microscopic algae of marine or non-marine origin tha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12P21/00
CPCA23L17/60A23V2002/00C12N1/12C12P7/6463A23V2250/202
Inventor 西尔万·德拉罗什马里·勒吕耶劳伦特·塞盖拉海克·耶罗施阿芒迪娜·德吕翁
Owner CORBION BIOTECH INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com