Bee pollen antioxidant milk and preparation method thereof
A technology of bee pollen and oxidized milk, which is applied in food ingredients as antimicrobial preservation, food science, food preservation, etc., can solve the problems that have not been reported in the literature and are not easy to accept, and achieve unique flavor, excellent tissue state, high The effect of commercial promotion value
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[0026] The preparation method of bee pollen total flavonoids extract is as follows: take buckwheat bee pollen as raw material, take the ethanol of concentration 75% as extraction solvent, according to the solid-liquid ratio is 1: 10, heat and reflux for 2 hours, centrifuge, take supernatant, filter residue Repeat the above operations, combine the supernatants twice, concentrate, and then freeze-dry to obtain a powder extract, that is, the bee pollen total flavonoids extract.
[0027] The total flavonoid content in bee pollen flavonoid-β-CD inclusion compound was 3.85mg / g.
[0028] Emulsion stabilizer is sodium carboxymethyl cellulose.
[0029] The preparation method of the above-mentioned bee pollen antioxidant milk provided by the embodiment of the present invention comprises the following steps:
[0030] Step 1, preparing bee pollen flavone-β-CD inclusion compound;
[0031] The preparation of bee pollen flavone-β-CD clathrate is carried out according to the following metho...
Embodiment 1
[0041] The preparation method of bee pollen total flavonoids extract in the present embodiment is: take buckwheat bee pollen as raw material, take the ethanol of concentration 75% as extraction solvent, be 1: 10g / L according to solid-liquid ratio, heat and reflux for 2 hours, centrifuge, Take the supernatant, then add the obtained filter residue to the extraction solvent again according to the above-mentioned operation method, and perform the steps of heating to reflux, centrifuging, and taking the supernatant; combine the supernatant twice, concentrate, and then freeze-dry to obtain a powder extract, namely The bee pollen total flavonoid extract. The freeze-drying was carried out in a freeze-drying machine, and the freeze-drying machine used was the LGJ-FD-1 freeze-drying machine of Xi'an Yuhui Instrument Co., Ltd.
[0042] Preparation of Bee Pollen Flavone-β-CD Inclusion Complex
Embodiment 2
[0044] Prepare bee pollen total flavonoids extract according to Example 1, use the prepared bee pollen total flavonoids extract as the core material, use β-cyclodextrin as the wall material, and use the ratio of 1:4 according to the mass ratio of the core and wall materials Weigh the bee pollen total flavonoids extract and β-cyclodextrin, then add distilled water to the β-cyclodextrin to prepare a saturated solution at 40°C. When preparing a 40°C saturated solution of β-cyclodextrin, the - Cyclodextrin is added in a ratio of 25ml of distilled water, and the total flavonoids extract of bee pollen is dissolved in an ethanol solution with a concentration of 75%, and slowly added dropwise to the β-CD saturated solution at 40°C, stirred at constant temperature for 2 hours, and cooled to room temperature , let stand at 4°C for 24 hours, then vacuum filter, rinse the filter residue with a small amount of absolute ethanol quickly, after the ethanol is evaporated, put the filter residue...
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