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Citric acid fermentation technology

A fermentation process, citric acid technology, applied in the direction of fermentation, microorganisms, biochemical equipment and methods, etc., to shorten the fermentation cycle, facilitate growth, and improve the effect of cultivation

Active Publication Date: 2016-03-09
RIZHAO JINHE BOYUAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at many technical bottlenecks encountered in the existing high-temperature drying corn citric acid fermentation and cultivation, the present invention provides a high-temperature drying corn citric acid fermentation and cultivation process, which is characterized in that during the cultivation process of citric acid seed tanks and fermentation tanks, through Add mixed nitrogen sources and trace elements to make up for the loss of high-temperature drying corn nutrients to achieve ideal cultivation effects; according to the different characteristics of bacterial growth and acid production stages, the present invention adjusts the type and proportion of nitrogen sources, and adds trace elements Elements to achieve the best cultivation effect

Method used

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  • Citric acid fermentation technology

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A citric acid fermentation process, its concrete steps are:

[0033] (1) Grind the high-temperature dried corn and mix it with 55°C warm water at a weight ratio of 1:2.6 to prepare the slurry. After stirring evenly, add high-temperature-resistant α-amylase at a ratio of 0.5kg amylase / t flour, and spray once (temperature 95°C), and then maintained for 1.5h, and then sprayed twice (temperature 105°C), adding amylase at a ratio of 0.3kg amylase / t flour, waiting for use after passing the iodine test, and the total sugar of the liquefied liquid is 22g / 100ml.

[0034] (2) Add the liquefied corn liquefied liquid I and ammonium sulfate into the seed tank, add water to constant volume, and prepare the seed tank culture medium that the total sugar is 10.2g / 100ml and the total nitrogen is 0.22g / 100ml, wherein the corn liquefied liquid I The ratio of nitrogen content to ammonium sulfate is 2.5:1, sterilized at 121°C for 30 minutes, kept under pressure and cooled down for later use;...

Embodiment 2

[0046] (1) Grind the high-temperature dried corn and mix it with 55°C warm water at a weight ratio of 1:2.8 to prepare the slurry. After stirring evenly, add high-temperature-resistant α-amylase at a ratio of 0.5kg amylase / t flour, and spray once (temperature 95°C), and then maintained for 1.5h, and then sprayed twice (at a temperature of 105°C), adding amylase at a ratio of 0.3kg amylase / t flour, waiting for use after passing the iodine test, and the total sugar of the liquefied liquid is 20.9g / 100ml .

[0047] (2) Add the liquefied corn liquefied liquid I and ammonium sulfate into the seed tank, add water to constant volume, and prepare a seed tank culture medium whose total sugar is 9.9g / 100ml and total nitrogen is 0.22g / 100ml, wherein the corn liquefied liquid I The ratio of nitrogen content to ammonium sulfate is 2.5:1, sterilized at 121°C for 30 minutes, kept under pressure and cooled down for later use; at the same time prepare trace element solution for seed tank: CuSO...

Embodiment 3

[0059] (1) Grind the high-temperature dried corn and mix it with 55°C warm water at a weight ratio of 1:3.0 to prepare the slurry. After stirring evenly, add high-temperature-resistant α-amylase at a ratio of 0.5kg amylase / t flour, and spray once (temperature 95°C), then maintain for 1.5h, and then spray twice (temperature 105°C), add amylase at the ratio of 0.3kg amylase / t flour, stand by after passing the iodine test, the total sugar of the liquefied liquid is 20.0g / 100ml .

[0060] (2) Add the liquefied corn liquefied liquid I and ammonium sulfate into the seed tank, add water to constant volume, and prepare the seed tank culture medium that the total sugar is 9.6g / 100ml and the total nitrogen is 0.22g / 100ml, wherein the corn liquefied liquid I The ratio of nitrogen content to ammonium sulfate is 2.5:1, sterilized at 121°C for 30 minutes, kept under pressure and cooled down for later use; at the same time prepare trace element solution for seed tank: CuSO 4 .7H 2 O50mg, Z...

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Abstract

The invention relates to the field of citric acid fermentation, in particular to a citric acid fermentation technology. The citric acid fermentation technology is characterized in that in a citric acid seed tank and a fermentation tank culture process, a mixed nitrogen source and microelements are added to compensate for the loss of nutritional ingredients of high-temperature dried corn in the high-temperature drying process to reach the ideal culture effect; the optimal culture effect is achieved by regulating the variety and ratio of the nitrogen source and adding the microelements according to the different characteristics of strains in the growing and acid production stages. After the technology is adopted, the seed age of seed culture can be shortened, fermentation stability can be improved, fermentation production intensity can be improved, the fermentation period can be shortened, the fermented sugar acid conversion rate and the equipment utilization rate can be increased, the source of citric acid fermentation raw materials is expanded, the competitiveness of the citric acid industry is improved, and then the economic benefits of an enterprise are improved.

Description

technical field [0001] The invention relates to the field of citric acid fermentation, in particular to a high-temperature drying corn citric acid fermentation process. Background technique [0002] Citric acid, also known as citric acid, is scientifically named 2-hydroxypropanetricarboxylic acid, 2-hydroxypropane-1,2,3-tricarboxylic acid (2-hydroxypropane-1,2,3-tricarboxylic acid), Molecular formula C 6 h 8 o 7 . It is a colorless transparent or translucent crystal, or granular, granular powder, odorless, although it has a strong sour taste, it is pleasant, slightly astringent, safe and non-toxic, and is widely used as a sour agent for food and beverages . The main raw materials for the production of citric acid are corn, dried melon, and cassava, among which corn is mostly used as raw material, and the corn production in Northeast my country accounts for about 30% of the country. The moisture content of corn is relatively high (25%-30%). At present, the acquisition co...

Claims

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Application Information

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IPC IPC(8): C12P7/48C12R1/685
Inventor 寇光智李昌涛陈祥军高晓彤刘长静蒋水星
Owner RIZHAO JINHE BOYUAN BIOCHEM
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