A compound microbial preparation for inhibiting anaerobic fermentation overacidification of vegetable waste
A technology of compound microorganisms and vegetable waste, applied in the field of compound microbial preparations, can solve problems such as high sugar content, collapse of biogas fermentation system, cumbersome operation, etc.
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Embodiment 3
[0075] The Bacillus subtilis (Bacillus subtilis) described in Example 3 is derived from the China Industrial Microorganism Culture Collection and Management Center, and the strain number is CICC 10020;
[0076] The Bacillus cereus (Bacillus cereus) described in Example 3 is derived from the China Industrial Microorganism Culture Collection Management Center, and the strain number is CICC 10042;
[0077] The Bacillus megaterium (Bacillus megaterium) described in Example 3 is derived from the China Industrial Microorganism Culture Collection and Management Center, and the strain number is CICC 20611;
[0078] The Bacillus licheniformis (Bacillus licheniformis) described in Example 3 is derived from the American Type Culture Collection, strain number: ATCC 14580;
[0079] The Pichia pastoris described in Example 3 is derived from the China Industrial Microorganism Culture Collection and Management Center, and the strain number is CICC 32806;
[0080] The Saccharomyces cerevisiae...
Embodiment 1
[0082] A method for preparing a compound microbial preparation for inhibiting anaerobic fermentation overacidification of vegetable waste, comprising the steps of:
[0083] (1) Bacillus subtilis (Bacillus subtilis), Bacillus cereus (Bacillus cereus), Bacillus megaterium (Bacillus megaterium), Bacillus licheniformis (Bacillus licheniformis) were subjected to activation culture, shake flask culture, seed culture and fermentation culture , the concentration of live bacteria was 2.0×10 9CFU / ml of Bacillus subtilis fermentation broth, Bacillus cereus fermentation broth, Bacillus megaterium fermentation broth and Bacillus licheniformis fermentation broth;
[0084] The activation conditions are: static culture at 37°C for 24 hours, and the activation medium is LB solid medium;
[0085] Shake flask culture conditions are: shaking culture at 37°C for 24 hours, rotation speed 180r / min, shake flask culture medium is LB liquid medium;
[0086] The seed culture conditions are all: shakin...
Embodiment 2
[0098] A method for preparing a compound microbial preparation for inhibiting anaerobic fermentation overacidification of vegetable waste, comprising the steps of:
[0099] (1) Bacillus subtilis, Bacillus cereus, Bacillus megaterium and Bacillus licheniformis were subjected to activation culture, shake flask culture, seed culture and fermentation culture respectively. , the concentration of viable bacteria was 1.0×10 9 CFU / ml of Bacillus subtilis fermentation broth, Bacillus cereus fermentation broth, Bacillus megaterium fermentation broth and Bacillus licheniformis fermentation broth;
[0100] The activation conditions are: static culture at 37°C for 24 hours, and the activation medium is LB solid medium;
[0101] Shake flask culture conditions are: shaking culture at 37°C for 24 hours, rotation speed 180r / min, shake flask culture medium is LB liquid medium;
[0102] The seed culture conditions are all: shaking culture at 37°C for 24 hours, the rotation speed is 180r / min, a...
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