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A compound microbial preparation for inhibiting anaerobic fermentation overacidification of vegetable waste

A technology of compound microorganisms and vegetable waste, applied in the field of compound microbial preparations, can solve problems such as high sugar content, collapse of biogas fermentation system, cumbersome operation, etc.

Active Publication Date: 2018-10-12
INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Vegetable waste has the characteristics of water content, high sugar content, perishability, and large compositional differences. When anaerobic fermentation is performed with it as raw material, the fermentation speed is usually fast because the substrate is easily utilized by the initial fermentation microorganisms, resulting in suboptimal fermentation. High-grade metabolites-organic acids accumulate rapidly in a short period of time. If these organic acids cannot be digested by downstream methanogenic archaea and other microorganisms in time, the fermentation system will be over-acidified, the pH will drop sharply, and the metabolism of most functional microorganisms Inhibited, leading to the collapse of the biogas fermentation system, unable to achieve normal production of biogas
In response to this problem, artificial dosing of alkaline substances can be used to adjust the pH, adding cow dung and other digestible substrates to change the material ratio, etc., but these solutions also have problems such as cumbersome operation and large restrictions on material sources. , in many cases it is not appropriate

Method used

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  • A compound microbial preparation for inhibiting anaerobic fermentation overacidification of vegetable waste
  • A compound microbial preparation for inhibiting anaerobic fermentation overacidification of vegetable waste

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Experimental program
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Effect test

Embodiment 3

[0075] The Bacillus subtilis (Bacillus subtilis) described in Example 3 is derived from the China Industrial Microorganism Culture Collection and Management Center, and the strain number is CICC 10020;

[0076] The Bacillus cereus (Bacillus cereus) described in Example 3 is derived from the China Industrial Microorganism Culture Collection Management Center, and the strain number is CICC 10042;

[0077] The Bacillus megaterium (Bacillus megaterium) described in Example 3 is derived from the China Industrial Microorganism Culture Collection and Management Center, and the strain number is CICC 20611;

[0078] The Bacillus licheniformis (Bacillus licheniformis) described in Example 3 is derived from the American Type Culture Collection, strain number: ATCC 14580;

[0079] The Pichia pastoris described in Example 3 is derived from the China Industrial Microorganism Culture Collection and Management Center, and the strain number is CICC 32806;

[0080] The Saccharomyces cerevisiae...

Embodiment 1

[0082] A method for preparing a compound microbial preparation for inhibiting anaerobic fermentation overacidification of vegetable waste, comprising the steps of:

[0083] (1) Bacillus subtilis (Bacillus subtilis), Bacillus cereus (Bacillus cereus), Bacillus megaterium (Bacillus megaterium), Bacillus licheniformis (Bacillus licheniformis) were subjected to activation culture, shake flask culture, seed culture and fermentation culture , the concentration of live bacteria was 2.0×10 9CFU / ml of Bacillus subtilis fermentation broth, Bacillus cereus fermentation broth, Bacillus megaterium fermentation broth and Bacillus licheniformis fermentation broth;

[0084] The activation conditions are: static culture at 37°C for 24 hours, and the activation medium is LB solid medium;

[0085] Shake flask culture conditions are: shaking culture at 37°C for 24 hours, rotation speed 180r / min, shake flask culture medium is LB liquid medium;

[0086] The seed culture conditions are all: shakin...

Embodiment 2

[0098] A method for preparing a compound microbial preparation for inhibiting anaerobic fermentation overacidification of vegetable waste, comprising the steps of:

[0099] (1) Bacillus subtilis, Bacillus cereus, Bacillus megaterium and Bacillus licheniformis were subjected to activation culture, shake flask culture, seed culture and fermentation culture respectively. , the concentration of viable bacteria was 1.0×10 9 CFU / ml of Bacillus subtilis fermentation broth, Bacillus cereus fermentation broth, Bacillus megaterium fermentation broth and Bacillus licheniformis fermentation broth;

[0100] The activation conditions are: static culture at 37°C for 24 hours, and the activation medium is LB solid medium;

[0101] Shake flask culture conditions are: shaking culture at 37°C for 24 hours, rotation speed 180r / min, shake flask culture medium is LB liquid medium;

[0102] The seed culture conditions are all: shaking culture at 37°C for 24 hours, the rotation speed is 180r / min, a...

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Abstract

The invention relates to a compound microbial preparation for inhibition of anaerobic fermentation over-acidification of vegetable wastes. The compound microbial preparation includes bacillus subtilis, bacillus cereus, bacillus megaterium, bacillus licheniformis, pichia pastoris, and saccharomyces cerevisiae. In an anaerobic fermentation environment, the microbial preparation has abilities of both producing alkaline substances and decomposing organic acids, can maintain acid-base balance of a vegetable waste anaerobic fermentation system, is well suitable for wet and dry anaerobic fermentation with the vegetable wastes as raw materials, and can guarantee smooth implementation of a biogas production process.

Description

technical field [0001] The invention relates to a compound microbial preparation for inhibiting anaerobic fermentation overacidification of vegetable waste, and belongs to the technical field of biogas additives. Background technique [0002] With the promotion and popularization of advanced agricultural production equipment and technologies such as new greenhouses and precision fertilization, as well as the introduction and improvement of many new vegetable varieties, my country's vegetable planting industry has achieved vigorous development, and a wide variety of vegetables has greatly enriched urban and rural areas. The masses' vegetable baskets have also significantly increased the economic income of farmers. However, it is worth noting that in the process of planting vegetables, it is inevitable to produce plant parts such as stems, leaves, and roots that are not suitable for consumption, and in the process of product transportation and marketing, some vegetables may als...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12P5/02C12R1/125C12R1/085C12R1/11C12R1/10C12R1/84C12R1/865
CPCY02E50/30
Inventor 付龙云王艳芹姚利夏江华杨光袁长波
Owner INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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