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ECMA (ecols composite microbial agents) preparation method

A composite microbial inoculum and a manufacturing method technology, applied in the field of bioengineering, can solve problems such as the surge in production costs, the destruction of micro-ecological balance, and the aggravation of soil-borne diseases, so as to reduce the occurrence of soil-borne diseases, inhibit growth and reproduction, and promote effect on growth

Inactive Publication Date: 2016-02-24
HEILONGJIANG KELE AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In order to meet the demand of population growth for production, agricultural production has long relied on a large number of chemical fertilizers and pesticides as the main measures to increase production. As a result, green manure and farmyard manure have been ignored, and the use of chemical fertilizers has increased. The cost of agricultural production has surged. At the same time, it also leads to soil compaction and fertilizer decline
Due to the loss and residue of chemical fertilizers and pesticides, the quality of agricultural products and human health are affected, causing environmental pollution, and at the same time destroying the original micro-ecological balance in the rhizosphere soil of crops, aggravating soil-borne diseases

Method used

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  • ECMA (ecols composite microbial agents) preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The first step is to cultivate the slant. Inoculate the strain Penicillium on the sterilized slant medium, and then put these test tubes into the incubator and cultivate them on the slant at 28°C for one week until the aerial spore piles turn green.

[0047] In the second step, shake flask strain culture.

[0048] The medium formula used: glucose 30g / l, KH 2 PO 4 6g / l, K 2 HPO 4 2.3g / l, (NH 4 ) 2 SO 4 3g / l, MgSO 4 ·7H 2 O0.2g / l, corn steep liquor 2g / l, adjust pH to 7.5.

[0049] In a 500ml Erlenmeyer flask equipped with a shaker flask strain culture medium, the filling volume is 100ml, inoculate the above-mentioned slant culture strain, and cultivate it for 26 hours at 150 rpm on a shaker at 30°C.

[0050] The third step is seed cultivation in liquid fermenter.

[0051] The medium formula used: glucose 120g / l, KH 2 PO 4 2g / l, K 2 HPO 4 2.5g / l, (NH 4 ) 2 SO 4 5g / l, MgSO 4 ·7H 2 00.2g / l, amino acid granule 8g / l, pH value is 7.5, prepares 350 liters of ...

Embodiment 2

[0060] The first step is to cultivate the slant. Inoculate the strain Penicillium on the sterilized slant medium, and then put these test tubes into the incubator and cultivate them on the slant at 30°C for one week until the aerial spore piles turn green.

[0061] In the second step, shake flask strain culture.

[0062] The medium formula used: glucose 35g / l, KH 2 PO 4 1g / l, K 2 HPO 4 1.33g / l, (NH 4 ) 2 SO 4 4g / l, MgSO 4 ·7H 2 O0.2g / l, corn steep liquor 2.5g / l, adjust pH to 7.2.

[0063] In a 500ml Erlenmeyer flask equipped with a shaker flask strain culture medium, the filling volume is 100ml, inoculate the above-mentioned slant culture strain, and cultivate for 26 hours at 150 rpm on a shaker at 32°C.

[0064] The third step is seed cultivation in liquid fermenter.

[0065] The medium formula used: glucose 100g / l, KH 2 PO 4 1.5g / l, K 2 HPO 4 2g / l, (NH 4 ) 2 SO 4 6g / l, MgSO 4 ·7H 2 00.2g / l, amino acid granule 10g / l, pH value is 8.0, prepares 350 liters of...

Embodiment 3

[0074] 1 ton of amino acid granules was mixed with 20 kg of ECMA to make amino acid bio-fertilizer, and the organic rice planting test was carried out. The comparative test of amino acid bio-fertilizer and inorganic fertilizer is shown in the table below. The planting results showed that applying 30kg of amino acid bio-fertilizer per mu at one time, without applying any fertilizer during the growth period, can still ensure strong plants, well-developed root system, dark green color, and no defertilization, and the yield level is basically the same as that of conventional fertilization.

[0075] Effect of Amino Acid Biofertilizer on Organic Rice Yield (2014)

[0076] Organic food rice yield measurement unit: kg / ㎡

[0077] deal with

average Yield per mu bio organic fertilizer 0.85 0.89 0.9 0.87 582.5 "733" organic fertilizer 0.82 0.80 0.83 0.817 544.7

[0078] Explanation: The actual yield is measured in the field, 3 point...

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Abstract

The invention discloses an ECMA (ecols composite microbial agents) preparation method. The method includes: inoculating purified penicillia to a slope of a potato culture medium, culturing, inoculating to a shake flask culture medium, performing shake-flask shake culture, inoculating to a liquid seed culture medium, culturing for 36-54 hours, sprinkling and inoculating to a solid fermentation medium, culturing at the temperature of 26-37 DEG C for 136-168 hours with a ventilation rate being 1:0.2-0.8, compounding prepared penicillium spore powder with bacillus subtilis to obtain a composite agriculatural microbial agent with functions of phosphate solubilizing and generating of various physiological activators. The utilization rate of phosphate in soil can be increased, soil-borne diseases can be prevented and reduced, and the efficacies of yield increasing and revenue increasing are achieved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a manufacturing method of ECMA composite microbial bacterial agent. Background technique [0002] In order to meet the demand of population growth for production, agricultural production has long relied on a large number of chemical fertilizers and pesticides as the main measures to increase production. As a result, green manure and farmyard manure have been ignored, and the use of chemical fertilizers has increased. The cost of agricultural production has surged. At the same time, it also leads to soil compaction and fertilizer decline. Due to the loss and residue of chemical fertilizers and pesticides, the quality of agricultural products and human health are affected, causing environmental pollution, and at the same time destroying the original micro-ecological balance in the rhizosphere soil of crops, aggravating soil-borne diseases. Soil is the nutrient source for the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F11/08A01N63/04A01P21/00C09K17/00C12R1/125C12R1/80C09K101/00A01N43/90A01N43/38A01N43/12
Inventor 李振刚
Owner HEILONGJIANG KELE AGRI CO LTD
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