Multiplex PCR detection kit for mink origin component identification and identification of mink, rabbit and dog components in animal products

Abstract

Belonging to the technical field of animal origin component molecular detection, the invention provides a multiplex PCR detection kit for mink origin component identification and identification of mink, rabbit and dog components in animal products. The kit provided by the invention includes mink, rabbit, dog and other species-specific primers, a reaction reagent, and positive control and blank control. The mink specific primer is a primer pair able to specifically amplify the nucleotide sequence shown as SEQ ID No.1. The primer provided by the invention is employed to amplify sample DNA, and the PCR amplification product is analyzed to determine whether the sample contains mink species origin ingredients, and at the same time the kit can identify whether rabbit origin or dog origin components are mixed, thus providing means for molecular identification of fur products. In addition, by means of multiplex PCR detection, mink origin, rabbit origin and dog origin components can be identified at one time. The invention establishes an effective method for identification of meat and fur adulteration and feed components. The method and kit have the advantages of simplicity, fastness, comprehensiveness, strong specificity, low cost, and wide applicability.

Description

technical field [0001] The invention relates to the field of animal molecular biology, in particular to the identification of mink-derived components and a multiplex PCR detection kit for mink, rabbit and dog components in animal products. Background technique [0002] Fur, also known as fur, refers to clothing made of animal fur. It may be one of the earliest clothing materials for humans. With the development of the times, fur has gradually become a symbol of luxury and wealth. Animals commonly used to make fur include mink, fox, rabbit, raccoon dog, beaver, etc., which are expensive and are one of the high-end winter clothing for women. The fur that people often say generally refers to mink fur. [0003] Mink belongs to Mammalia, Carnivora, Mustelidae, Weasel in animal taxonomy, and is one of the small and precious fur animals. Mink fur is one of the three treasures of Northeast China. It is known as "the king of fur" and is called "soft gold" abroad. Mink fur is a ki...

AI Technical Summary

Problems solved by technology

The collars of mink fur bought in the market may be made of ordinary rabbit fur or dog fur through deep processing, and ordinary consumers cannot tell the difference at all.

In addition, there are also many unscrupulous traders who process or mix mink carcass meat, internal organs or bones into edible meat products and feed, deceiving consumers and disrupting market order

Using traditional morphological methods to identify the source of fur, meat or feed ingredients, human error is large, easy to misjudge, and the accuracy rate is low

At present, in the published patents of our country, there is a method for identifying sable and pine mink (Li Bo, Xu Yanchun. Primers for amplifying the cytochrome b gene of mink species and identifying sable and pine mink [P], Chinese patent: CN101792816A ,2010-8-4.), there are also methods to detect mink-derived components, but the animal species involved are less (Li Mingcheng, Zhang Lihua, Wang Bingmei, Sun Hongxia. Mink heart DNA detection kit and identification method [P], China Patent: CN101434990A, 2009-5-20.) or the method is complicated and takes a long time (Li Guangyu, Sun Weili, Zhao Jiaping. A group of amplification primers for detecting mink-derived components [P], Chinese patent: CN102643912B, 2014-1 -8), can not easily and quickly identify mink and other animal fur

There are few reports on the identification of mink-derived components in mink meat products and feed

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