Biological profiling and plugging agent, preparation method and application
A plugging agent and biological technology, applied in the direction of chemical instruments and methods, drilling compositions, etc., can solve the problems of direct breakthrough or channeling of bacterial liquid, poor application effect, poor formation homogeneity, etc., and achieve non-degradable , good salt resistance, and the effect of improving the swept volume
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Embodiment 1
[0025] The biological clogging agent in this embodiment is composed of the following components in mass percentage: 10% dry powder of encapsulated strains, 10% nutrient base, 1% hydrogen peroxide, and the balance is water.
[0026] The preparation steps of the encapsulated strain dry powder are as follows:
[0027] 1) Xanthomonas campestris (purchased from China Center for Type Culture Collection, strain preservation number: CCTCCAB96030, genus name: Xanthomonas, species name: campestris, other preservation unit number: ATCC9924, preservation date: August 10, 1996 Day) after activation, inoculate into conventional LB medium (or potato medium), ferment and cultivate for 36h to the logarithmic phase, centrifuge for 20min at a rotating speed of 3000r / min, discard the supernatant, and get sediment and glycerol according to the volume ratio of 1: 1. Mix evenly. The mixture is first allowed to stand at a temperature of 4°C for 60 minutes, then at a temperature of -30°C and -80°C for...
Embodiment 2
[0037] The biological clogging agent in this embodiment is composed of the following components in mass percentage: 5% of encapsulated strain dry powder, 0.4% of nutrient base, 0.1% of hydrogen peroxide, and the balance is water. The preparation method is the same as in Example 1.
Embodiment 3
[0039] The biological clogging agent in this embodiment is composed of the following components in mass percentage: 5% of encapsulated strain dry powder, 5% of nutrient base, 0.5% of hydrogen peroxide, and the balance is water. The preparation method is the same as in Example 1.
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