Application of lycopalhine A in preparation of drugs for treating or preventing yellow fever virus infections

A technology of yellow fever virus and medicine, which is applied in the direction of antiviral agents, pharmaceutical formulas, and resistance to vector-borne diseases, etc., to achieve the effect of strong inhibitory activity and outstanding substantive characteristics

Inactive Publication Date: 2016-01-27
ZIBO DINGLI PATENT INFORMATION CONSULTING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no cases reported in China, but this does not make us relax our vigilance against the virus, because China's climate and geographical environment are complex, and there are many types of mosquitoes, which have the conditions for transmission. At the same time, with the increasing frequency of international exchanges, YFV It is very likely that it will be introduced into China

Method used

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  • Application of lycopalhine A in preparation of drugs for treating or preventing yellow fever virus infections
  • Application of lycopalhine A in preparation of drugs for treating or preventing yellow fever virus infections
  • Application of lycopalhine A in preparation of drugs for treating or preventing yellow fever virus infections

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: the preparation of the compound LycopalhineA tablet involved in the present invention:

[0014] Get 20 grams of compound Lycopalhine A and add 180 grams of conventional excipients for tablet preparation, mix well, and make 1000 tablets with a conventional tablet press.

Embodiment 2

[0015] Embodiment 2: the preparation of the compound LycopalhineA capsule involved in the present invention:

[0016] Get 20 grams of compound Lycopalhine A and add conventional auxiliary materials such as starch 180 grams for preparing capsules, mix well, and pack into capsules to make 1000 capsules.

[0017] The following pharmacodynamic experiments will further illustrate its drug activity.

experiment example 1

[0019] Toxicity test of A.LycopalhineA on Vero cells

[0020] Vero cells (African green monkey kidney cells) are susceptible cells to YFV.

[0021] The experimental steps are as follows:

[0022] 1: Inoculate Vero cells: use DMEM medium containing 10% (v / v) fetal bovine serum to make a single cell suspension, inoculate 1000-10000 cells per well into a 96-well cell culture plate, and inoculate a volume of 100ul per well ;

[0023] 2: Cultivate Vero cells: at 37°C, 5% (v / v) CO2 culture conditions, culture for 2 days;

[0024] 3: Add Lycopalhine A: suck out the DMEM medium in each well, add 100ul to each well and dilute to the corresponding concentration (0uM, 0.4uM, 1.2uM) with DMEM medium containing 10% (v / v) fetal calf serum , 3.7uM, 11uM, 33uM, 100uM, 300uM) of LycopalhineA, the control wells were added without adding 100ul of DMEM medium containing 10% (v / v) fetal bovine serum;

[0025] 4: Coloring: After culturing for 48 hours, add 10ul of MTT solution to each well, con...

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PUM

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Abstract

The invention discloses an application of lycopalhine A in the preparation of drugs for treating or preventing yellow fever virus infections. Lycopalhine A is effective in inhibiting the proliferation of yellow fever virus, has little toxicity on cells, can be further developed into drugs for treating diseases caused by these virus infections and has a promising application prospect. The application of the lycopalhine A in the preparation of drugs for treating yellow fever virus infections is disclosed for the first time; since its skeleton type is novel and the lycopalhine A is has unexpected activity in inhibiting yellow fever virus, chances that other compounds given any implication are zero; the lycopalhine A has outstanding substantive features and has significant progress in use for controlling the yellow fever virus infections.

Description

technical field [0001] The present invention relates to a new application of compound Lycopalhine A, in particular to the application of Lycopalhine A in the preparation of medicines for treating or preventing yellow fever virus infection. Background technique [0002] Yellow fever virus (yellowfevervirus, YFV) is a member of the Flavivirus genus of the family Flaviviridae. Yellow fever virus causes yellow fever, an acute infectious disease. Yellow fever has been designated as a quarantine infectious disease internationally, and China has also designated it as a Class A infectious disease. In 1648, an epidemic of yellow fever was first confirmed in the weekly YUCATAN peninsula. From the 17th to the 19th century, the disease was brought to Europe and North America through transportation. Within two centuries, yellow fever became one of the most serious plagues in some parts of the United States, Africa, and Europe, causing a large number of deaths. Since the 20th century, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/529A61P31/14
CPCY02A50/30
Inventor 田丽华
Owner ZIBO DINGLI PATENT INFORMATION CONSULTING CO LTD
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