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New use of benserazide hydrochloride reverse cholesterol transport way

A technology of benserazide hydrochloride and cholesterol, which is applied in the field of biomedicine and can solve problems such as hepatic high-density lipoprotein cholesterol uptake blockage

Inactive Publication Date: 2016-01-27
CHINA PHARM UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of data show that SRB1 is a decisive regulator of HDL metabolism. It is found that in SRB1 knockout mice, the uptake of high-density lipoprotein cholesterol mediated by SR-BI in the liver is almost completely blocked. At the same time, many literatures reported that the liver The inhibitory effect of SRB1 expression on atherosclerosis is most likely due to its role as a regulator of the final step in reverse cholesterol transport, potentially enhancing cholesterol influx into the liver

Method used

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  • New use of benserazide hydrochloride reverse cholesterol transport way
  • New use of benserazide hydrochloride reverse cholesterol transport way
  • New use of benserazide hydrochloride reverse cholesterol transport way

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] 1) Cells stained with Oil Red O are washed with PBS→fixed with 4% paraformaldehyde for 10~20min-→washed with PBS→stained with Oil Red O for 15min→washed with water→observed under microscope

[0024] 2) cell-basedELISA detection

[0025] After the cells are incubated for 24 hours, discard the culture medium, wash and dry, fix the cells with 4% paraformaldehyde for 20-30 minutes, rinse with PBS / TritonX-100 for 5 minutes × 3 times, 0.6% H 2 O 2 Incubate with PBS / Tritonx-100 for 20-30min to block endogenous peroxidase, rinse with PBS / TritonX-100 for 5min×3 times, block with 10% BSA at 37°C for 1h40min, and incubate with 50u15% BSA diluted SRB1 primary antibody at 4°C Overnight, rinse with PBS / TritonX-100 for 5min×3 times, incubate with 50ul 5% BSA diluted horseradish peroxidase labeled secondary antibody at 37℃ for 1h, rinse with PBS / TritonX-100 for 5min×3 times, rinse with PBS for 5min×3 times, Add 200ul TMB color developing solution to each well and keep it at 37°C for 30 minu...

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Abstract

The invention discloses the promotion of benserazide hydrochloride on a reverse cholesterol transport (RCT) way process so as to play a role in prevention and treatment of atherosclerosis. By establishing foam macrophage and liver cell model under an atherosclerosis condition, the invention studies the influence of benserazide hydrochloride on the expression of two crucial speed limiting receptors (ATP binding cassette transporter A1, scavenger receptor B1) in the reverse cholesterol transport (RCT) process, and finds that benserazide hydrochloride especially has very significant promoting effect on liver SRB1 and then promotes the last step of reverse cholesterol transport (RCT), thus having very important significance in prevention and treatment of atherosclerosis diseases. The study conforms to the development direction of China medicine modernization, and has important social significance and potential economic benefits in the fundamental research field and application research field.

Description

Technical field [0001] The present invention relates to the field of biomedicine, and mainly relates to the effect of benserazide hydrochloride on the expression of two important rate-limiting receptors of the reverse cholesterol transport pathway (RCT), thereby promoting the reverse cholesterol transport pathway (RCT) to reverse atherosclerosis . Background technique [0002] Atherosclerosis (AS) has become an important cause of human death. Among them, it is generally believed that high-density lipoprotein (HDL) has the effect of inhibiting atherosclerosis, mainly through its mediated reverse cholesterol transport pathway (RCT). to realise. [0003] The reverse cholesterol transport pathway (RCT) mainly promotes cholesterol efflux from macrophages through a variety of ways, and promotes lipidation of ApoA-1 and HDL, and then mature HDL is transported to the liver for excretion and secretion, thereby hindering atherosclerosis. The ATP-binding cassette transporter A1 (ABCA1) is m...

Claims

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Application Information

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IPC IPC(8): A61K31/165A61P9/10
Inventor 何书英方婷欢余新超
Owner CHINA PHARM UNIV
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