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Rosa davurica pall extract and applications thereof

A kind of extract, the technology of thorn rose, applied in the thorn rose extract and its application field

Inactive Publication Date: 2016-01-20
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no reports about the whitening and anti-aging effects of the extract of Rosa japonica

Method used

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  • Rosa davurica pall extract and applications thereof
  • Rosa davurica pall extract and applications thereof
  • Rosa davurica pall extract and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation of embodiment 1 Rosa japonica extract

[0038] (1), preparation of Rosa japonica flower extract: add 70% ethanol 1000mL after pulverizing 100g of Rosa japonica flower, ultrasonically extract 2 times at room temperature, each 1 hour (also can adopt stirring extraction, reflux extraction etc. commonly used Extraction method, preferably ultrasonic extraction), then filter, the solvent of the filtrate is concentrated to 50ml under reduced pressure, adsorbed with AB-8 resin column, and eluted with deionized water, 30% ethanol, 70% ethanol and 95% ethanol successively, respectively After the collection, the solvent was distilled off under reduced pressure to obtain a dry solid, which was weighed to obtain 2.95 g of the 30% ethanol part, 9.89 g of the 70% ethanol part, and 2.45 g of the 95% ethanol part of the rose flower.

[0039] (2), preparation of Rosa japonica fruit extract: add 70% ethanol 1000mL after pulverizing 100g of Rosa japonica fruit, ultrasonical...

Embodiment 2

[0043] Example 2 Research on Antioxidant Activity of Rosa japonica Extract

[0044] With the extract of Rosa japonica prepared in Example 1, its free radical scavenging activity was measured by the DPPH method, with VC as a reference. The determination procedure is: the extract of Rosa japonica is prepared into an aqueous solution with deionized water respectively, pipette 2 mL into a 10 mL stoppered test tube, add 2 mL of DPPH ethanol solution (2×10 -4 mol / L), fully mix, stand at room temperature, measure the absorbance A of 517nm wavelength with a spectrophotometer after 30min 517 (with ethanol as a reference); simultaneously measure the absorbance A after mixing 2mLDPPH solution with 2mL ethanol c , and the absorbance A after mixing 2mL extract with 2mL ethanol solution 0 , measured in parallel three times, averaged, and calculated the free radical scavenging rate according to the following formula.

[0045] Free radical scavenging rate = (1-(A 517 -A 0 ) / A c )×100%. ...

Embodiment 3

[0051] Example 3 Research on the Inhibition of Elastase by the Extract of Rosa japonica

[0052] The extracts of Rosa japonica prepared in Example 1 were respectively dissolved in deionized water to prepare an aqueous solution for measuring elastase inhibitory activity. The assay method was carried out according to the literature (Am.J.Pharmacol.Toxicol., 2009, 4, 127-129), and the assay steps were: add 10 μL sample solution and 130 μL containing 1.015 mM reaction substrate Succ-Ala-Ala- Ala-p-nitroanilide 0.1M Tris-HCl buffer solution (pH8.0), incubate at 25°C for 5 minutes, add 15μL elastase solution (0.5U / ml), continue to incubate at 25°C 30min, then measure the absorbance A at 410nm wavelength with a microplate reader 410 . The sample aqueous solution was replaced with deionized water, and the absorbance was also measured as a reference solution. The test results are shown in Table 2.

[0053] The formula for calculating the inhibition rate of elastase activity is: inhi...

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Abstract

The present invention provides a rosa davurica pall extract and applications thereof, wherein the total flavone content in the rosa davurica pall extract is 0.7-6.46, and the rosa davurica pall extract is extracted from the whole rosa davurica pall plant, the rosa davurica pall flower, the rosa davurica pall fruit or the rosa davurica pall root. According to the present invention, the rosa davurica pall extract has effects of good whitening and good anti-aging, and can be used in the fields of cosmetics, health food and medicine, especially in cosmetics.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, and in particular relates to an extract of Rosa japonica and application thereof. Background technique [0002] Whitening and anti-aging cosmetics have always been the pursuit and yearning of women, and they are also eternal topics in the beauty industry. In recent years, with the development of social economy and the progress of science and technology, people's demand for cosmetics has also increased. [0003] The color of human skin is related to the types and quantities of pigments present in the skin, and melanin has the greatest influence on skin color. Regarding the formation of skin melanin, current research shows that it is mainly caused by biochemical reactions in melanocytes, that is, tyrosine generates dopaquinone under the action of tyrosinase as a catalyst, and then catalyzed by enzymes or Non-enzymatic oxidation forms melanin. Regarding the whitening active substances tha...

Claims

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Application Information

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IPC IPC(8): A61K36/738A61K8/97A23L1/29A61Q19/02A61Q19/08A61P17/16A61P17/18
Inventor 方兆华沈忠海摩根·多斯桑托斯宋肖洁李慧陈明华曹平
Owner JALA GROUP CORPORATION
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