Method for extracting high-molecular weight tannin from persimmons

A high-molecular-weight, persimmon technology, applied in the field of extracting high-molecular-weight tannins, can solve problems such as the inhibition of Streptococcus mutans by no persimmon tannins, and achieve the effect of inhibiting Streptococcus mutans

Inactive Publication Date: 2016-01-06
GUILIN MINGXING BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Bacillus subtilis is completely different from Streptococcus mutans, and there is no report that persimmon tannins can be used to inhibit Streptococcus mutans

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Peel and crush the persimmons, add 70v% ethanol 10 times the weight of the persimmons, extract at 60°C for 1 hour, combine the extracts, filter, and take the filtrate for later use;

[0017] 2) Concentrate the filtrate, add 95v% ethanol, the amount of ethanol added is 2 times of the concentrated solution, leave it overnight, centrifuge, and take the supernatant;

[0018] 3) Pass the supernatant through AB-8 macroporous resin, wash the column with water until it is colorless, then elute with ethanol, and collect the ethanol eluate;

[0019] 4) Concentrate the ethanol eluent to recover the solvent to obtain the extract, add water to dissolve, the amount of water added is 3 times the weight of the extract, filter, take the filtrate and pass it through a hollow fiber membrane with a molecular weight cut-off of 4000-5000, and collect the retentate for later use ;

[0020] 5) Pass the above-mentioned retentate through a hollow fiber membrane with a molecular weight cut-of...

Embodiment 2

[0022] 1) Peel and crush the persimmons, add 80v% ethanol 20 times the weight of the persimmons, extract 3 times at 80° C. for 3 hours each time, combine the extracts, filter, and take the filtrate for later use;

[0023] 2) Concentrate the filtrate, add 95v% ethanol, the amount of ethanol is 4 times that of the concentrated solution, leave it overnight, centrifuge, and take the supernatant;

[0024] 3) Pass the supernatant through AB-8 macroporous resin, wash the column with water until it is colorless, then elute with ethanol, and collect the ethanol eluate;

[0025] 4) Concentrate the ethanol eluent to recover the solvent to obtain the extract, add water to dissolve, the amount of water added is 5 times the weight of the extract, filter, take the filtrate and pass it through a hollow fiber membrane with a molecular weight cut-off of 4000-5000, and collect the retentate for later use ;

[0026] 5) Pass the above-mentioned retentate through a hollow fiber membrane with a mol...

Embodiment 3

[0028] 1) Peel and crush the persimmons, add 75v% ethanol 15 times the weight of the persimmons, extract twice at 70°C for 2 hours each time, combine the extracts, filter, and take the filtrate for later use;

[0029] 2) Concentrate the filtrate, add 95v% ethanol, the amount of ethanol added is 3 times of the concentrated solution, leave it overnight, centrifuge, and take the supernatant;

[0030] 3) Pass the supernatant through AB-8 macroporous resin, wash the column with water until it is colorless, then elute with ethanol, and collect the ethanol eluate;

[0031] 4) Concentrate the ethanol eluent to recover the solvent to obtain the extract, add water to dissolve, the amount of water added is 4 times the weight of the extract, filter, take the filtrate and pass it through a hollow fiber membrane with a molecular weight cut-off of 4000-5000, and collect the retentate for later use ;

[0032] 5) Pass the above-mentioned retentate through a hollow fiber membrane with a molecu...

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PUM

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Abstract

The invention discloses a method for extracting high-molecular weight tannin from persimmons. The method includes the following steps that 1, the persimmons are peeled and smashed, ethyl alcohol is added for extraction, extract is filtered, and filtrate is taken for use; 2, the filtrate is concentrated, 95V% ethyl alcohol is added, the mixture is placed over night and then centrifuged, and supernate is taken; 3, the supernate is made to pass through AB-8 macroporous resin, treated by a washing pillar to become colorless and then eluted through ethyl alcohol, and ethyl alcohol eluant is collected; 4, the ethyl alcohol eluant is concentrated, solvent is recovered, extractum is obtained, the extractum is added with water to be dissolved and then filtered, filtrate is taken and made to pass through a hollow fiber film with the molecular weight cut-off of 4000-5000, and cut-off liquid is collected for use; 5, the cut-off liquid is made to pass through a hollow fiber film with the molecular weight cut-off of 10000-12000, permeating liquid is collected and dried, and then the high-molecular weight tannin powder is obtained. The extracted high-molecular weight tannin has an excellent inhibiting effect on streptococcus mutans and can be used for preventing caries.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for extracting high molecular weight tannins from persimmons. Background technique [0002] Persimmon (Persimmon DiospryroskakiL) is rich in tannins in the pulp. Studies have shown that the total phenols of persimmon have strong antioxidant properties and protein-binding capabilities, and can inhibit human lymphocytic leukemia, lower blood pressure, remove free radicals in the body, and significantly inhibit various enzymes in the venom of Agkistrodon halys, cobras, and five-step snakes and toxin activity. It is currently reported that persimmon extract (active ingredients are flavonoids, triterpenoids and hydrolyzable tannins) has shown a certain drug resistance to Bacillus subtilis, but the drug resistance is not stable. But Bacillus subtilis is completely different from Streptococcus mutans, and there is no report that persimmon tannin can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H99/00A61P31/04A61P1/02
Inventor 文继承蒋元勇朱昂辉雍小军易华李丽金
Owner GUILIN MINGXING BIOTECH
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