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Sensing device, and sensing system and sensing method using the same

A micro-sensing and reaction technology, applied in the direction of measuring devices, laboratory containers, chemical instruments and methods, etc., can solve the problems of complex manufacturing process and difficult implementation, so as to reduce statistical errors, reduce errors, and reduce kinetics Analyzing the effect of time

Active Publication Date: 2015-12-23
ACAD SINICA +1
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Problems solved by technology

However, even if the detection time can be reduced from several hours to several minutes by the above methods, the main disadvantages of these methods are that they are difficult to implement and the manufacturing process is relatively complicated.

Method used

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  • Sensing device, and sensing system and sensing method using the same
  • Sensing device, and sensing system and sensing method using the same
  • Sensing device, and sensing system and sensing method using the same

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experiment example

[0059] Materials and Reagents

[0060] Biotinylated tris(ethylene glycol)undecylthiol (HS-(CH)n-OEG-biotin) was purchased from Nanoscience Instruments (USA). 11-mercapto-1-undecanol (MUD) (HS-(CH2)11-OH), bovine serum albumin (bovineserumalbumin, BSA) was purchased from ACROS Organics (France). Anti-immunoglobulin anti-mouse IgG and AlexaFluor647-conjugated monoclonal mouse anti-rabbit IgG were purchased from JacksonImmuoResearch (UK). AlexaFluor488 conjugated goat anti-rabbit antibody was purchased from Invitrogen, Inc. All proteins were diluted in phosphate-buffered saline (10 mM phosphate-buffered saline, 0.138M NaCl, 0.0027M KCl, pH 7.4), which was purchased from Sigma-Aldrich (France). And five kinds of composition used to prepare rigid polydimethylsiloxane, ethylene polydimethylsiloxane copolymer (vinylPDMScopolymer), ethylene modified silica Q resin (vinylmodifiedsilicaQresin), platinum-divinyl tetra Methyldisiloxane (platinum-divinyltetramethyldisiloxane), hydrosila...

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Abstract

A sensing device applied to an analyte molecule of a liquid sample and a buffer flow has at least one first inlet, at least one second inlet, a micro-flow channel, and at least one immobilization element. The first inlet is for inputting the liquid sample. The second inlet is for inputting the buffer flow. The micro-flow channel communicates with the first inlet and the second inlet. The immobilization element is for immobilizing sensing molecules for the analyte molecules. The analyte sample flow and the buffer flow, in the reverse direction, in the micro-flow channel enable the association and disassociation kinetics to be obtained. The present invention further provides a sensing system and a sensing method using the sensing device.

Description

technical field [0001] The present invention relates to a sensing device of biologically functionalized nano-slits combined with a fluorescence microscope, a sensing system and a sensing method using the same, so as to perform no-washing, real-time, and usable under simple and low-cost setting conditions. High-speed sensing kinetics (both binding and dissociation kinetics) studies of labeled molecules. Background technique [0002] Kinetic monitoring of protein interactions provides insight into the cellular functions of these proteins and is also key to developing potential diagnostic tests and biotherapeutics. Surface Plasmon Resonance (SPR) and Quartz Crystal Microbalance (QCM) are two existing standard commercial technologies used in the field of pharmaceuticals and life sciences to provide real-time information on molecular interactions without labels. detection. However, these detection techniques require high-cost dedicated sensor surfaces and the integration of opt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/68
CPCB01L3/5027G01N33/557B01L2300/0636B01L2300/0877G01N21/6428G01N21/6458G01N2021/6439G01N33/54366G01N21/763G01N33/54373
Inventor 周家复席瑞林以立派特马·蒂拉佩尼屈
Owner ACAD SINICA
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