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Artificial feeding method of cotton aphid parasitoid wasp-Lysiphlebia japonica

A cotton aphid wasp and artificial technology, which is applied to the field of artificial breeding of cotton aphid parasitoids, can solve the problems of not being able to collect cotton aphids, aphids running and crawling, loss of cotton aphid wasps, etc. The effect of high rate and control of bee spawning time

Inactive Publication Date: 2015-12-23
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no relevant report on the artificial breeding method of Aphids gossypii. The closest method is the breeding method of Aphids aphids. The method used is to directly graft adult aphids on the seedlings, and after a few days, the adult aphids will breed small aphids. Re-introduction of parasitoids, because parasitoids have a certain selectivity to the age of the host, resulting in a low parasitism rate
Due to its small size, its host aphids can secrete honeydew. Putting Aphids gossypii in beekeeping cages for parasitism, the parasitism rate is very low, and part of Aphis gossypii is secreted by aphids at the bottom of beekeeping cages The honeydew sticks to death, causing a serious loss of Aphids gossypii, and the existing large-area breeding method is not suitable for indoor small-area artificial breeding
Moreover, after the cotton aphid is parasitized, it will send out an alarm pheromone, causing the aphid to run around and crawl around, and the parasitized cotton aphid cannot be collected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The artificial rearing method of embodiment 1 Aphids gossypii

[0023] The method comprises the steps of:

[0024] 1. Take stiff aphids from the field and put them into beekeeping cages planted with blooming zucchini for the nectar of Aphids gossypii. The filter paper is sealed, and a layer of filter paper is placed at the bottom. One day after the aphids emerge from the bees, the female and male bees mate naturally, and the mated peaks are inserted into the fresh-keeping box with the aphids the next day. Moisten the bottom of the fresh-keeping box with water, spread cotton leaves flatly in the fresh-keeping box, connect the aphids of the 2nd to 3rd instar, and insert the aphids of cotton at a ratio of 1:50 (that is, Aphids gossypii: Aphis gossypii = 1:50), 25% honey-moistened absorbent cotton is placed in the fresh-keeping box, and the fresh-keeping box is sealed with a plastic wrap, and the plastic wrap is tightly packed with small holes to ensure ventilation, and th...

Embodiment 2

[0032] The constant temperature rearing test of embodiment 2 Aphids gossypii

[0033] 1. Take stiff aphids from the field, put them into beekeeping cages planted with blooming zucchini, and feed nectar to Aphids gossypii wasps. At the same time, put absorbent cotton moistened with 25% honey. The upper part of the beekeeping cage is sealed with filter paper, and the bottom is Put a layer of filter paper, one day after the stiff aphids emerge from the bees, the female bees and male bees will mate naturally, and the mated peaks will be inserted into the fresh-keeping box with aphids the next day.

[0034] 2. After 12 hours of parasitism by Aphids gossypii, remove the parasitoids, and the parasitoids can be inserted into a new fresh-keeping box to re-parasitize; use the double-layer cotton leaf method to feed the parasitized aphids, and the feeding conditions are light 14h / dark 10h, temperature 26°C, humidity 80%.

[0035] 3. After the parasitic aphids are raised at 26°C for 4-5 ...

Embodiment 3

[0042] Embodiment 3 does not adopt the test of double-layer cotton leaf method to raise Aphidius gossypii

[0043] 1. Take stiff aphids from the field, put them into beekeeping cages planted with blooming zucchini, and feed nectar to Aphids gossypii wasps. At the same time, put absorbent cotton moistened with 25% honey. The upper part of the beekeeping cage is sealed with filter paper, and the bottom is Put a layer of filter paper, one day after the stiff aphids emerge from the bees, the female bees and male bees will mate naturally, and the mated peaks will be inserted into the fresh-keeping box with aphids the next day.

[0044] 2. After 12 hours of parasitization by the cotton aphid, remove the parasitic wasp, and the parasitic wasp can be inserted into a new fresh-keeping box to parasitize again; the parasitized cotton aphid is kept in the fresh-keeping box, and there are 18% of the A layer of cotton leaves was laid on the top of the agar medium, and the fresh-keeping box ...

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Abstract

The invention provides an artificial feeding method of cotton aphid parasitoid wasp-Lysiphlebia japonica. According to the method, on the basis of biological characteristics of the Lysiphlebia japonica and cotton aphids, the Lysiphlebia japonica is inoculated with aphids at the preference age of the Lysiphlebia japonica, the Lysiphlebia japonica is fed through stepped temperature change, the parasitized aphids are fed with a double-layer cotton leaf method, the phenomenon that the parasitized aphids run and climb about due to the fact that the aphids give out alarm pheromones is avoided, the emergence time of the Lysiphlebia japonica can be effectively controlled, the growth period of the Lysiphlebia japonica can be shortened, and the lives of adult Lysiphlebia japonica can be prolonged. When the Lysiphlebia japonica is fed with the method, the parasitic rate of the Lysiphlebia japonica is high, the loss is low, the Lysiphlebia japonica is fed step by step, and feeding conditions can be controlled step by step. The method is convenient to operate and low in cost, indoor continuous feeding of the Lysiphlebia japonica can be realized, and a good foundation is laid for large-scale propagation and feeding of the Lysiphlebia japonica.

Description

technical field [0001] The invention relates to an artificial breeding method for parasitic wasps of cotton aphids, in particular to an artificial breeding method for parasitic wasps of cotton aphids. Background technique [0002] Cotton aphid (Aphisgossypii Glover) is a common pest widely distributed in countries all over the world. According to records, there are 600 species of host plants in 76 families of cotton aphids in the world, among which cotton and Cucurbitaceae melons are the main resident hosts (secondary hosts, summer hosts) of cotton aphids (Zhang Guangxue and Zhong Tiesen, 1983; Blackman, 2000 ). Due to the short reproductive cycle and large reproductive capacity of cotton aphid, once the conditions are suitable, the damage can break out in a short period of time. In addition, cotton aphids cause harm by migrating, spreading and changing hosts for food, and at the same time spread virus diseases of various vegetable crops such as melons. Especially in rece...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 高雪珂崔金杰张帅吕丽敏王春义雒珺瑜李春花
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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