Breeding method of a new cytoplasmic male sterile line of round red radish

Abstract

The invention relates to a method for cultivating a new cytoplasmic male sterile line of round red-skinned radish, comprising the following steps: selecting radish seeds of the female parent sterile line 262A and the male parent inbred line XYH-3-1-5 for the same period of time Sowing; one week after the emergence of the female and male radish seeds, thin the seedlings; after the radish seedlings reach 50‑60 days, they are planted in the isolation net room; Before flowering, bees are used for pollination; after the radish seed plants wither, the bees are removed, and the male parent inbred line XYH-3-1-5 is pulled out; Threshing; the harvested radish seeds are the new cytoplasmic male sterile line (262A×XYH‑3‑1‑5)A of round red radish. The new sterile line cultivated by the cultivation method of the invention has beneficial effects such as strong stress resistance, vigorous growth of seed plants and the like.

Description

technical field [0001] The invention relates to a method for cultivating a new cytoplasmic male sterile line of round red-skinned radish, which belongs to the field of plant breeding. Background technique [0002] Our country started the research and utilization of radish male sterility in the early 1970s, found abundant radish male sterility genetic resources, and prepared the first generation of excellent hybrids, which were used in production. Such as Jinhuatai 48A CMS, 77-01A CMS, 262A CMS and 401A CMS, etc., wherein 262A CMS is a widely used radish cytoplasmic male sterile line ("Radish Male Sterility Cytoplasmic Identification") and Classification" Zhang Li et al. "Chinese Vegetables" 2006 No. 6, "Research Progress in Radish Breeding" Chen Baogang et al. "Hebei Agricultural Sciences" 16 (1)), its main features are mosaic leaves, red veins, short round radish, The skin is light red, the flesh is white, the stamens degenerate and the pistils are normal during flowering,...

AI Technical Summary

Problems solved by technology

Seeds of the sterile line were obtained by crossing with its completely identical maintainer line 262B (only different in flowering fertility). Due to the single cytoplasm and long-term isolation and hybridization, the vitality of the sterile line decreased, and the specific manifestations were weak growth and resistance to Poor stress resistance, susceptibility to downy mildew, yellowing and shedding of flower buds during flowering, low pod setting rate, low seed production yield, and poor seed plumpness. Generally, the normal male sterile line seed production yield is 50-75kg, while using 262A The yield during seed production is only 20-30kg, and less than 80% of the seeds are fully formed. In case of extreme weather conditions, such as long-term drought or rainy weather, the seeds may even fail to be harvested, which affects the application of the sterile line and its hybrid varieties Promotion

[0003] The main reason for the decline in the viability of the cytoplasmic male sterile line 262A: the male sterile line and the maintainer line are completely identical except for the flowering fertility, and the seed acquisition method is similar to the principle of self-seed retention, and the cytoplasm is homogeneous and other reasons caused the stress resistance of the strains to be weak