A Fast Phase Distortion Compensation Method Based on Downsampled Phase Distortion Principal Component Analysis

Abstract

The invention discloses a rapid phase distortion compensation method based on downsampling phase distortion principal component analysis. The rapid phase distortion compensation method comprises the steps of: photographing a hologram by using a camera, extracting +1 order spectrum after acquiring frequency spectra of the hologram, removing other frequency spectra, and translating the +1 order spectrum to the center of the frequency spectra; extracting frequency spectra of phase distortion components from the frequency spectra, and extracting a downsampling phase diagram of an object through inverse Fourier transform; extracting the first principal component of the phase diagram through singular value decomposition, and fitting to obtain parabolic equations in x and y directions by utilizing a least square method; and fitting again to obtain accurate parabolic equations in the x and y directions after eliminating error points; carrying out sub-pixel interpolation on parabolas in the x and y directions to obtain an upsampling distortion phase diagram; and acquiring a compensated phase diagram by subtracting the distortion phase diagram from an original phase diagram of the object.

Description

technical field [0001] The invention belongs to the optical imaging correction technology, in particular to a fast phase distortion compensation method based on down-sampling phase distortion principal component analysis. Background technique [0002] As a new type of coherent measurement and imaging technology, digital holography has the biggest advantage of being able to simultaneously and independently obtain quantitative amplitude information and phase information of objects. Phase information is particularly important when performing quantitative detection of almost transparent objects such as biological cells. However, in order to obtain the phase image of the object accurately, the phase distortion in the reproduced image must be corrected first, which requires the knowledge of various parameters in the experiment, such as the recording distance, the angle between the object parameters, and the magnification of the microscope objective lens Wait. At the same time, p...

AI Technical Summary

Problems solved by technology

However, most of the above-mentioned methods for phase distortion compensation through post-calculation have a large amount of calculation, and the calculation time of fitting methods such as Zernike polynomial fitting or least square surface fitting decreases with the increase in the size of the captured hologram. How to achieve high-precision and fast phase distortion compensation has become a technical problem in digital holographic microscopic imaging

Images