Orthogonal flow and sandwich hepatic cell bioreactor

Abstract

The invention discloses an orthogonal flow and sandwich hepatic cell bioreactor which comprises a culturing barrel, an upper cover, a lower cover and a plurality of culturing units. Each culturing unit comprises a silica gel seal, a runner piece and a hepatic cell culture slice from bottom to top, wherein the hepatic cell culture slice comprises a round slice frame and two semi-transparent films attached to the upper surface and the lower surface of the round slice frame respectively; a plurality of first liquid passing holes are formed in the circumference of the round slice frame; the silica gel seal and the runner piece are of ring shapes with the outer diameters equal to that of the round slice frame; the two opposite sides of the circumferences of the silica gel seal and the runner piece are each provided with a protruding piece protruding in the inner diameter direction; a plurality of second liquid passing holes are formed in the circumferences of the corresponding protruding pieces. Blood passes through the first liquid passing holes in one sides of the hepatic cell culture slices, the silica gel seals and the runner pieces to form blood channels connected in series. Hepatic cell culture media pass the first liquid passing holes in the other two sides of the hepatic cell culture slices and the second liquid passing holes in the two opposite sides of the silica gel seals and the runner pieces to form hepatic cell culture medium channels.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and medical equipment, and in particular relates to an orthogonal movement and sandwich liver cell bioreactor. Background technique [0002] The in vitro culture system of hepatocytes, referred to as artificial liver, refers to the in vitro biological reaction system in which artificially cultured hepatocytes are used as the basic structure. The hepatocyte culture system in vitro is connected with the human blood circulation pathway. Provide extracorporeal liver support therapy for patients with liver diseases such as acute liver failure. [0003] At present, the main problems of artificial liver are: the density and scale of cell culture are not ideal, the material exchange performance between hepatocytes and plasma is not high, and it cannot provide an ideal microenvironment for hepatocytes, the oxygenation is not good, and the metabolites of hepatocytes It cannot be ruled out, the mate...

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Problems solved by technology

[0003] At present, the main problems of artificial liver are: the density and scale of cell culture are not ideal, the material exchange performance between hepatocytes and plasma is not high, and it cannot provide an ideal microenvironment for hepatocytes, the oxygenation is not good, and the metabolites of hepatocytes Unable to rule out, unsatisfactory substance transport in nutrient solution, no immune barrier, difficult to operate

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