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Application of Cephaloziellins H in preparation of prostate cancer treating medicine

A technology of cephaloziellinsh and 1.cephaloziellinsh, which can be applied in the directions of drug combination, antitumor drug, medical formula, etc., can solve the problem that there is no report on the activity of prostate cancer, etc.

Inactive Publication Date: 2015-12-16
刘高志
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is no report on the activity of this compound in the treatment of prostate cancer

Method used

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  • Application of Cephaloziellins H in preparation of prostate cancer treating medicine
  • Application of Cephaloziellins H in preparation of prostate cancer treating medicine
  • Application of Cephaloziellins H in preparation of prostate cancer treating medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: Isolation, Preparation and Structure Confirmation of CephaloziellinsH

[0013] The preparation method of Cephaloziellins H is the same as that reported in the literature (Secondary Metabolites from the Chinese Liverwort Cephaloziellakiaeri, J. Nat. Prod., 2013, 76, 1700-1708).

[0014] Confirmation of structure: colorless crystal (methanol), melting point 150-153°C. According to HR-ESI-MS, the molecular formula is C 20 h 22 o 7 , with an unsaturation of 10. H NMR spectrum data δ H (ppm, DMSO-d 6 , 600MHz): H-1 (1.81, m), H-1 (1.65, m), H-2 (2.23, 2H, m), H-3 (6.74, dd, J=3.6, 3.0), H- 6 (4.62, dd, J=10.2, 6.6), H-7 (1.95, dd, J=13.8, 6.6), H-7 (2.08, dd, J=13.8, 10.2), H-9 (2.48, dd , J=9.0, 7.2), H-11 (2.53, dt, J=13.0, 7.2), H-11 (2.19, ddd, J=13.0, 9.0, 6.0), H-12 (5.36, dd, J= 7.2, 6.0), H-14 (6.27, br, s), H-15 (7.33, br, s), H-16 (7.35, br, s), H-17 (1.42, s), H-19 (1.26, s); C NMR spectrum data δ C (ppm, DMSO-d 6 , 150Hz): 33.3 (CH 2 , 1-C)...

Embodiment 2

[0015] Embodiment 2: Pharmacological action test of Cephaloziellins H

[0016] 1. Materials and Instruments

[0017] Prostate cancer cell line PC3 (ArCC-CRL-1435), prostate cancer cell line DU145 (ATCC-HTB-81). CephaloziellinsH is self-made, and the HPLC normalized purity is greater than 98%. It is prepared into a storage solution with a concentration of 1.0 g / L with dimethylsulfoxide (DMSO) for future use. The CCK-8 kit is a product of Japan Tongren Chemical Research Institute. RPMI-1640 medium was purchased from Gibco. Fetal bovine serum (FBS) was purchased from Hyelone Company. Penicillin / streptomycin is a product of Shanghai Pioneer Pharmaceuticals. Trypsin was purchased from Huamei Bioengineering Company. Dimethyl sulfoxide (DMSO) was purchased from Shanghai Huashun Bioengineering Company. Agarose (Agarose), dithiothreitol (DTT), phenylmethylsulfonphthalein fluoride (PMSF), and tetramethylethylenediamine (TEMED) are products of Sigma. Sodium dodecylsulfonate (SDs),...

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Abstract

The invention discloses application of Cephaloziellins H in preparation of prostate cancer treating medicine, and belongs to the field of medicine. Researches show that Cephaloziellins H has an effect of inhibiting prostate cancer cell lines PC3 and DU145, and the effect has a concentration--time dependent relation, so that Cephaloziellins H can be further developed into preparation of prostate cancer treating medicine.

Description

technical field [0001] The invention relates to a new application of compound Cephaloziellins H, in particular to the application of Cephaloziellins H in the preparation of medicaments for treating prostate cancer. Background technique [0002] Rui-JuanLi et al. isolated and purified the compound Cephaloziellins H for the first time, and published the results in the famous Journal of Natural Products (Secondary Metabolites from the Chinese Liverwort Cephaloziellakiaeri, J. Nat. Prod., 2013, 76, 1700-1708). [0003] At present, there is no report about the activity of this compound in treating prostate cancer. Contents of the invention [0004] The purpose of the present invention is to provide a medical application of Cephaloziellins H. [0005] The above-mentioned purpose is achieved through the following technical solutions: [0006] Application of CephaloziellinsH in the preparation of a drug for treating prostate cancer, the chemical structural formula of the Cephalo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P35/00C07D493/04
Inventor 刘高志
Owner 刘高志
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