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Method for establishing nile tilapia merit female pure line

A technology of Nile tilapia and excellent traits, applied in the field of germplasm improvement of Nile tilapia, can solve the problems of decline of good traits of breeds, improvement of difficult genetic traits, increase of genetic diversity of parents, etc.

Inactive Publication Date: 2015-12-16
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0002] Oni hybrid tilapia (the offspring of the male parent of Oria tilapia mated with the female parent of Nile tilapia) is one of the main species of tilapia cultured in my country. After nearly 20 to 30 years of breeding, especially the parent The over-utilization of the parental species increases the genetic diversity of the parents. It is difficult to obtain precise improvements in the genetic traits through the traditional phenotypic trait selection method, resulting in the reproductive rate of the female parent (Nile tilapia) and the produced Oni hybrid. There are different degrees of differences and differentiation in the male rate and stress resistance of tilapia fry, and there is a decline trend in the excellent traits of the species

Method used

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Examples

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Embodiment Construction

[0018] 1. Parent selection:

[0019] During the breeding season in June, among the existing species of Nile tilapia, 1-year-old individuals are selected for breeding, and the breeding is carried out in cages, with 60 fish per cubic meter in size, and 2 fish in each cage 3 Nile tilapia males and 3 Nile tilapia females, after that, check the spawning situation of the Nile tilapia females every other week (eggs are contained in the mouth of the female fish), and the number of eggs laid is 1500 The above females of Nile tilapia are picked out and concentrated in special ponds for breeding as spare broodstock, and the females of Nile tilapia below 1500 are discarded. During the selection process, the female parents are appropriately supplemented to achieve the required number of selections. One month later, a total of 400 female Nile tilapia were picked out.

[0020] In June of the second year, 30 2-year-old red tilapia males were prepared. From 400 Nile tilapia females, 60 with ...

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Abstract

The invention discloses a method for establishing a nile tilapia merit female pure line. Only XX female tilapia is generated through gynogenetic egg chromosome doubling, false male tilapia (XX) obtained after sex reversion can carry out fertilization normally with the female tilapia, so that fry of the later generation are all female tilapia, and in other words, the female pure line is achieved. The nile tilapia merit female pure line is established through the method of parent selecting, maturation accelerating of parents, gynogenesis, sex reversion and inbreeding, and the merits of nile tilapia as reproduction female parents are effectively maintained.

Description

technical field [0001] The invention belongs to the germplasm improvement technology of Nile tilapia. In particular, it is a method for establishing a pure line of female Nile tilapia through parental selection, ripening, gynogenesis, sex reversal of gynogenetic offspring, and male-female mating after reversal. Background technique [0002] Oni hybrid tilapia (the offspring of the male parent of Oria tilapia mated with the female parent of Nile tilapia) is one of the main species of tilapia cultured in my country. After nearly 20 to 30 years of breeding, especially the parent The over-utilization of the parental species increases the genetic diversity of the parents. It is difficult to obtain precise improvements in the genetic traits through the traditional phenotypic trait selection method, resulting in the reproductive rate of the female parent (Nile tilapia) and the produced Oni hybrid. The male rate, stress resistance and other traits of tilapia fry have different degree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 徐跑何杰强俊
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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