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Glycyl-L-tyrosine chiral isomer separation and detection method

A technology of chiral isomers and tyrosine, which is applied in the field of analytical chemistry, can solve problems such as detection of glycyl-L-tyrosine isomers that have not yet been retrieved, and achieve good separation, high sensitivity, and results Accurate and reliable results

Inactive Publication Date: 2015-12-09
北京紫萌医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant literature report on the detection of glycyl-L-tyrosine isomers

Method used

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  • Glycyl-L-tyrosine chiral isomer separation and detection method
  • Glycyl-L-tyrosine chiral isomer separation and detection method
  • Glycyl-L-tyrosine chiral isomer separation and detection method

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Experimental program
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Effect test

Embodiment 1

[0017] (1) Instruments and conditions

[0018] Chromatographic column: crown ether chiral column ( CR(+), 150×4.6mm, 5.0μm)

[0019] Mobile phase: Perchloric acid aqueous solution at pH 1.3

[0020] Detection wavelength: 220nm

[0021] Column temperature: 30°C

[0022] Flow rate: 0.7ml / min

[0023] Injection volume: 10μl

[0024] (2) Experimental steps

[0025] System suitability solution: Take appropriate amount of glycyl-D-tyrosine and glycyl-L-tyrosine, add mobile phase to dissolve and dilute to make about 0.5% glycyl-D-tyrosine per 1ml μg, glycyl-L-tyrosine 0.5mg solution,

[0026] Take the system suitability solution and analyze it under the above conditions. see results figure 1 , the retention time of glycyl-D-tyrosine is 25.012min, and the retention time of glycyl-L-tyrosine is 27.497min.

Embodiment 2

[0028] (1) Instruments and conditions

[0029] Chromatographic column: crown ether chiral column ( CR(+), 150×4.6mm, 5.0μm)

[0030] Mobile phase: Perchloric acid aqueous solution at pH 1.2

[0031] Detection wavelength: 220nm

[0032] Column temperature: 35°C

[0033] Flow rate: 0.8ml / min

[0034] Injection volume: 10μl

[0035] (2) Experimental steps

[0036] System suitability solution: Take appropriate amount of glycyl-D-tyrosine and glycyl-L-tyrosine, add mobile phase to dissolve and dilute to make about 0.5% glycyl-D-tyrosine per 1ml μg, glycyl-L-tyrosine 0.5mg solution,

[0037] Take the system suitability solution and analyze it under the above conditions. see results figure 2 , the retention time of glycyl-D-tyrosine is 21.360min, and the retention time of glycyl-L-tyrosine is 23.369min.

Embodiment 3

[0039] (1) Instruments and conditions

[0040] Chromatographic column: crown ether chiral column ( CR(+), 150×4.6mm, 5.0μm)

[0041] Mobile phase: Perchloric acid aqueous solution at pH 1.3

[0042] Detection wavelength: 220nm

[0043] Column temperature: 40°C

[0044] Flow rate: 0.9ml / min

[0045] Injection volume: 10μl

[0046] (2) Experimental steps

[0047] System suitability solution: Take appropriate amount of glycyl-D-tyrosine and glycyl-L-tyrosine, add mobile phase to dissolve and dilute to make about 0.5% glycyl-D-tyrosine per 1ml μg, glycyl-L-tyrosine 0.5mg solution,

[0048] Take the system suitability solution and analyze it under the above conditions. see results image 3 , the retention time of glycyl-D-tyrosine is 19.164min, and the retention time of glycyl-L-tyrosine is 20.965min.

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Abstract

The present invention provides a glycyl-L-tyrosine chiral isomer separation and detection method, belonging to the field of analytical chemistry. In the method, a chiral crown ether column is used as a chromatographic column, a perchloric acid water solution is used as a mobile phase, separation and detection of glycyl-L-tyrosine and glycyl-L-tyrosine chiral isomer glycyl-D-tyrosine can be can be achieved by high performance liquid chromatography method.

Description

technical field [0001] The invention relates to a method for separating and detecting chiral isomers of glycyl-L-tyrosine, which belongs to the field of analytical chemistry. technical background [0002] Glycyl-L-tyrosine is an important source of tyrosine, and it is one of the dipeptide components in the compound amino acid (15) dipeptide (2) injection. This product overcomes the problem of poor solubility of tyrosine in water , which enriches the formula of amino acid injection. Its chemical name is glycyl-L-tyrosine dihydrate, and its chemical structure is as follows: [0003] [0004] It can be seen from the structural formula that there are chiral isomers in this product, that is, glycyl-D-tyrosine. The optical purity of the product is strictly controlled by a specific analytical method, and a reasonable and feasible chiral isomer is formulated. The impurity limit is an important part of the quality control of glycyl-L-tyrosine, and has important practical signifi...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 尚宝虎刘晓春
Owner 北京紫萌医药科技有限公司
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