Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Graphene derivative based glucose oxidase electrode and preparation method thereof

A technology of glucose oxidase and graphene, which is applied in the field of electrochemical analysis and detection, can solve the problems of poor conductivity of graphene oxide and low electrode reusability, and achieve the effects of improving detection sensitivity, facilitating reusability, and good stability

Inactive Publication Date: 2015-12-09
NANJING UNIV OF SCI & TECH
View PDF3 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

JuanTian et al. directly used graphene oxide to build electrodes for signal amplification. However, graphene oxide has a high solubility in water. In water system detection, the electrode’s reusability is low. In addition, graphene oxide has poor conductivity (TianJ, YuanPX, ShanD, et al. Biosensing platform based on graphene oxide via self-assembly induced by synergic interactions [J]. Analytical biochemistry, 2014, 460: 16-21.)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Graphene derivative based glucose oxidase electrode and preparation method thereof
  • Graphene derivative based glucose oxidase electrode and preparation method thereof
  • Graphene derivative based glucose oxidase electrode and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0019] The preparation method of the glucose oxidase electrode based on graphene derivative, concrete steps are as follows:

[0020] Step 1, the base electrode is polished, cleaned and dried;

[0021] Step 2. Dissolve equal mass of dopamine hydrochloride and graphene oxide in Tris-HCl buffer solution at pH 8.5, disperse evenly, stir and react, then centrifuge and wash, dry to obtain graphene derivative / dopamine complex, and then compound The graphene derivative / dopamine complex solution with a concentration of 0.5 to 3 mg / mL was prepared by dissolving the dopamine;

[0022] Step 3, drop-coating the graphene derivative / dopamine complex solution obtained in step 2 on the treated conductive electrode substrate;

[0023] Step 4, use the electrode obtained in step 3 as the working electrode, Ag / AgCl as the reference electrode, the platinum electrode as the counter electrode, and the dopamine phosphate buffer solution with a concentration of 4mM and a pH value of 7.5 as the electro...

Embodiment 1

[0029] Step 1, the platinum electrodes are respectively made of Al with a particle size of 0.3 μm and 0.05 μm 2 o 3 The suspension was polished into a mirror surface on suede, then ultrasonicated with absolute ethanol and ultrapure water for 2 minutes each, and dried in a desiccator for later use;

[0030] Step 2. Dissolve 30 mg of dopamine hydrochloride in PH=8.5 Tris-HCl solution, then add graphene oxide of equal mass, disperse evenly by ultrasonic, stir and react for 4 hours, centrifuge at 14,000 rpm and 4°C for 30 minutes, remove the supernatant, and Centrifuge and wash three times with sub-distilled water, then dry the precipitate, dissolve the complex in sub-distilled water, and ultrasonically disperse to prepare a 0.5 mg / mL graphene derivative / dopamine complex solution;

[0031] Step 3, drop 10 μ L of the graphene derivative / dopamine complex solution obtained in step 2 on the treated platinum electrode;

[0032] Step 4, use the electrode obtained in step 3 as the work...

Embodiment 2

[0039] Step 1, the platinum electrodes are respectively made of Al with a particle size of 0.3 μm and 0.05 μm 2 o 3The suspension was polished into a mirror surface on suede, then ultrasonicated with absolute ethanol and ultrapure water for 2 minutes each, and dried in a desiccator for later use;

[0040] Step 2. Dissolve 30 mg of dopamine hydrochloride in PH=8.5 Tris-HCl solution, then add graphene oxide of equal mass, disperse evenly by ultrasonic, stir and react for 4 hours, centrifuge at 14,000 rpm and 4°C for 30 minutes, remove the supernatant, and Centrifuge and wash with sub-distilled water three times, then dry the precipitate, dissolve the complex in sub-distilled water, and ultrasonically disperse to prepare a 1 mg / mL graphene derivative / dopamine complex solution;

[0041] Step 3, drop 10 μ L of the graphene derivative / dopamine complex solution obtained in step 2 on the treated platinum electrode;

[0042] Step 4, use the electrode obtained in step 3 as the working...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a graphene derivative based glucose oxidase electrode and a preparation method thereof. The electrode is sequentially composed of a conductive electrode substrate, a graphene derivative / dopamine composite layer, a polydopamine layer, a nanogold array, a 4-mercaptophenylboronic acid layer, and a glucose oxidase layer. According to the invention, dopamine is employed to reduce graphene oxide partially into reduced graphene oxide to form a graphene derivative / dopamine composite layer gathering on the surface of the conductive electrode substrate, also electropolymerization of a layer of polydopamine is carried out on the surface of the graphene derivative / dopamine composite layer, self-assembly of nanogold particles is performed on the platform, and then 4-mercaptophenylboronic acid is loaded to the nanogold array so as to fix glucose oxidase. The glucose oxidase electrode has the advantages of simple preparation method, low detection limit, high sensitivity and high enzyme bonding strength, and can realize sensitive detection of glucose.

Description

technical field [0001] The invention belongs to the technical field of electrochemical analysis and detection, and in particular relates to a graphene derivative-based glucose oxidase electrode and a preparation method thereof. Background technique [0002] Diabetes mellitus, a metabolic disease characterized by hyperglycemia due to insulin deficiency, is one of the leading causes of death and disability. Diagnosis and control of diabetes requires constant monitoring of blood glucose levels, so thousands of diabetics need to check their blood sugar every day. How to carry out reliable and close blood sugar control is still a topic worthy of research. Among them, the glucose electrochemical biosensor plays an important role in blood glucose monitoring, and the current-type enzyme electrode based on the glucose oxidase immobilized on the electrode is widely used in the research of the glucose electrochemical biosensor. [0003] In recent years, glucose detection has graduall...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/327
Inventor 单丹孙文秀袁培新
Owner NANJING UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com