Preparation method and application of a cationic modified glycoprotein microbial flocculant
A microbial flocculant and glycoprotein technology, applied in the field of water treatment, can solve problems such as secondary pollution of the environment, and achieve the effect of simplifying the addition process, simplifying the addition process, and reducing the content
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Embodiment 1
[0031] This embodiment provides a preparation method of a cationic modified glycoprotein microbial flocculant, the method comprising the following steps:
[0032] Step 1, preparing glycoprotein microbial flocculant:
[0033] Step 1.1, prepare seed solution:
[0034] Pick a ring of bacteria from the preservation slant of Klebsiella NY1 strain and insert it into beef extract peptone liquid medium for culture to obtain OD 600nm Klebsiella NY1 seed solution of 1.82 ± 0.08;
[0035] Step 1.2, Klebsiella NY1 fermentation to produce flocculant:
[0036] 100 mL of fermentation culture was sterilized at 121° C. for 30 min. Under a sterile environment, draw 2 mL of Klebsiella NY1 seed solution and add it to the fermentation medium. Under the conditions of 30°C and 160r / min, shake and cultivate at constant temperature for 72 hours to obtain the crude product of glycoprotein microbial flocculant;
[0037] Step 1.3, the purification method of the crude product of glycoprotein microbia...
Embodiment 2
[0064] This embodiment provides a preparation method of a cationic modified glycoprotein microbial flocculant, the method comprising the following steps:
[0065] Step 1, preparing glycoprotein microbial flocculant:
[0066] The specific content of step 1 is basically the same as that of Example 1, except that the stabilizing agent in step 1.3 is potassium chloride.
[0067] Step 2, cationic modification of glycoprotein microbial flocculant:
[0068] Mix 0.02mol of 3-chloro-2-hydroxypropyltrimethylammonium chloride (CTA) with NaOH solution with a concentration of 0.5mol / L at a molar ratio of 1:1.6, let it react for 10min, then add 10g of NY1 Pure flocculant produced by bacteria. The mixture was stirred at room temperature for 0.5 h, put into an incubator and allowed to react at 60° C. for 1 h to obtain a mixture of cation-modified glycoprotein microbial flocculants.
[0069] Step 3, purification of cationized glycoprotein microbial flocculant:
[0070] Completely dissolve ...
Embodiment 3
[0086] This embodiment provides a preparation method of a cationic modified glycoprotein microbial flocculant, the method comprising the following steps:
[0087] Step 1, preparing glycoprotein microbial flocculant:
[0088] The specific content of step 1 is basically the same as that of Example 1, except that the stabilizing agent in step 1.3 is sodium sulfate.
[0089] Step 2, cationic modification of glycoprotein microbial flocculant:
[0090] Mix 0.02mol of 3-chloro-2-hydroxypropyltrimethylammonium chloride (CTA) with 1mol / L NaOH solution at a molar ratio of 1:0.8, let it react for 10min, then add 10g of NY1 bacteria The flocculant produced is pure. The mixture was stirred at room temperature for 2 hours, put into a thermostat to react at 60° C. for 1 hour, and a mixture of cation-modified glycoprotein microbial flocculants was obtained.
[0091] Step 3, purification of cationized glycoprotein microbial flocculant:
[0092] Completely dissolve the mixture obtained in t...
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